Establishment and characterization of a human juvenile bone marrow-derived mesenchymal stem/stromal cell line under advanced culture conditions for osteogenic differentiation

Title (eng)
Establishment and characterization of a human juvenile bone marrow-derived mesenchymal stem/stromal cell line under advanced culture conditions for osteogenic differentiation
Author
Julia Moldaschl
Author
Sofia Danilchenko
Author
Isita Sagar
Author
Vivian-Pascal Brandt
Author
Heidrun Holland
Author
Stephan Handschuh
Author
Martin Glösmann
Author
Stefan Toegel
Author
Tobias May
Author
Dominik Egger
Author
Cornelia Kasper
Abstract (eng)
Human mesenchymal stem/stromal cells (MSC) from juvenile donors (juvMSC) are crucial for studying bone development and for modeling pediatric skeletal diseases. However, the limited availability of these cells and the lack of physiologically relevant in vitro models hinder preclinical research. To address these issues, we established and characterized a new human bone marrow-derived MSC line under advanced culture conditions. Primary MSC from a 12-year-old donor in good health were immortalized via lentiviral transduction using a library of expansion genes. The resulting clone, C15 juvMSC, retained key features of MSC, including typical morphology, high proliferation rate, expression of stemness surface markers, and trilineage differentiation in a 3D format. Spectral karyotyping confirmed genomic stability without chromosomal aberrations. In 3D spheroid cultures, the C15 juvMSC demonstrated strong osteogenic potential, as evidenced by mineralization and alkaline phosphatase (ALP) activity. However, they exhibited a distinct differentiation pattern compared to primary cells. Overall, the C15 juvMSC line exhibits stable, scalable, and physiologically relevant characteristics, making it a valuable model for studying osteogenesis and for in vitro research on pediatric bone disorders.
Keywords (eng)
3D CultureCell LineJuvenileMesenchymal Stem/Stromal CellOsteogenic Differentiation
Type (eng)
journal article
Language
[eng]
Persistent identifier
https://phaidra.vetmeduni.ac.at/o:5333
DOI
10.3389/fbioe.2025.1719466
Is in series
Title (eng)
Frontiers in Bioengineering and Biotechnology
Volume
13
ISSN
2296-4185
Issued
2026
Number of pages
17
Publication
Frontiers Media SA
Version type (eng)
version of record (published version)
Date issued
2026
Access rights (eng)
open access
License
CC BY 4.0 International
Rights statement (eng)
© 2026 Moldaschl, Danilchenko, Sagar, Brandt, Holland, Handschuh, Glösmann, Toegel, May, Egger and Kasper