https://phaidra.vetmeduni.ac.at/o:5333 Establishment and characterization of a human juvenile bone marrow-derived mesenchymal stem/stromal cell line under advanced culture conditions for osteogenic differentiation Moldaschl, Julia Julia Moldaschl Danilchenko, Sofia Sofia Danilchenko Sagar, Isita Isita Sagar Brandt, Vivian-Pascal Vivian-Pascal Brandt Holland, Heidrun Heidrun Holland Handschuh, Stephan Stephan Handschuh 0000-0002-2140-7892 University of Veterinary Medicine Vienna Glösmann, Martin Martin Glösmann 0000-0002-2094-3247 University of Veterinary Medicine Vienna Toegel, Stefan Stefan Toegel May, Tobias Tobias May Egger, Dominik Dominik Egger Kasper, Cornelia Cornelia Kasper Frontiers Media SA journal article open access eng Human mesenchymal stem/stromal cells (MSC) from juvenile donors (juvMSC) are crucial for studying bone development and for modeling pediatric skeletal diseases. However, the limited availability of these cells and the lack of physiologically relevant in vitro models hinder preclinical research. To address these issues, we established and characterized a new human bone marrow-derived MSC line under advanced culture conditions. Primary MSC from a 12-year-old donor in good health were immortalized via lentiviral transduction using a library of expansion genes. The resulting clone, C15 juvMSC, retained key features of MSC, including typical morphology, high proliferation rate, expression of stemness surface markers, and trilineage differentiation in a 3D format. Spectral karyotyping confirmed genomic stability without chromosomal aberrations. In 3D spheroid cultures, the C15 juvMSC demonstrated strong osteogenic potential, as evidenced by mineralization and alkaline phosphatase (ALP) activity. However, they exhibited a distinct differentiation pattern compared to primary cells. Overall, the C15 juvMSC line exhibits stable, scalable, and physiologically relevant characteristics, making it a valuable model for studying osteogenesis and for in vitro research on pediatric bone disorders. 3D Culture Cell Line Juvenile Mesenchymal Stem/Stromal Cell Osteogenic Differentiation http://creativecommons.org/licenses/by/4.0/ https://phaidra.vetmeduni.ac.at/api/object/o:5333/download 10.3389/fbioe.2025.1719466 https://phaidra.vetmeduni.ac.at/o:605 application/pdf Frontiers in Bioengineering and Biotechnology issn:2296-4185 VoR Frontiers in Bioengineering and Biotechnology 13 3.69 MB 2026