Julia Moldaschl Julia Moldaschl Sofia Danilchenko Sofia Danilchenko Isita Sagar Isita Sagar Vivian-Pascal Brandt Vivian-Pascal Brandt Heidrun Holland Heidrun Holland Stephan Handschuh Stephan Handschuh Martin Glösmann Martin Glösmann Stefan Toegel Stefan Toegel Tobias May Tobias May Dominik Egger Dominik Egger Cornelia Kasper Cornelia Kasper Frontiers Media SA 2026 Human mesenchymal stem/stromal cells (MSC) from juvenile donors (juvMSC) are crucial for studying bone development and for modeling pediatric skeletal diseases. However, the limited availability of these cells and the lack of physiologically relevant in vitro models hinder preclinical research. To address these issues, we established and characterized a new human bone marrow-derived MSC line under advanced culture conditions. Primary MSC from a 12-year-old donor in good health were immortalized via lentiviral transduction using a library of expansion genes. The resulting clone, C15 juvMSC, retained key features of MSC, including typical morphology, high proliferation rate, expression of stemness surface markers, and trilineage differentiation in a 3D format. Spectral karyotyping confirmed genomic stability without chromosomal aberrations. In 3D spheroid cultures, the C15 juvMSC demonstrated strong osteogenic potential, as evidenced by mineralization and alkaline phosphatase (ALP) activity. However, they exhibited a distinct differentiation pattern compared to primary cells. Overall, the C15 juvMSC line exhibits stable, scalable, and physiologically relevant characteristics, making it a valuable model for studying osteogenesis and for in vitro research on pediatric bone disorders. PDFDocument eng 2026-07-14T12:41:09.198648878Z 2026 3D Culture Cell Line Juvenile Mesenchymal Stem/Stromal Cell Osteogenic Differentiation 3873971 b application/pdf http://creativecommons.org/licenses/by/4.0/