Advancing standardization of diagnostics and antimicrobial susceptibility testing for pathogenic mycoplasmas of livestock origin: insights from the MyMIC network

Title (eng)
Advancing standardization of diagnostics and antimicrobial susceptibility testing for pathogenic mycoplasmas of livestock origin: insights from the MyMIC network
Author
Maryne Jaÿ
Author
Tiina Autio
Author
Claire A.M. Becker
Author
Jade Bokma
Author
Cécile Boland
Author
Filip Boyen
Author
Salvatore Catania
Author
Katarzyna Dudek
Author
Emma Hurri
Author
Anneke Feberwee
Author
Miklós Gyuranecz
Author
Inna Lysnyansky
Author
Lucía Manso-Silván
Author
Franca Möller Palau-Ribes
Author
Ana S. Ramirez
Author
Anne Ridley
Author
Joachim Spergser
Author
Paola K. Vaz
Author
Nadeeka Wawegama
Author
Jeanine Wiegel
Author
Annet E. Heuvelink
Author
Gudrun Overesch
Author
Anne V. Gautier-Bouchardon
Author
Florence Tardy
Author
Sara M. Klose
Author
Marco Bottinelli
Abstract (eng)
Mycoplasma infections pose a significant economic burden and represent a serious health and welfare concern for the livestock sector. Their control often requires repeated antimicrobial treatments. Antimicrobial susceptibility testing (AST) procedures for veterinary mycoplasmas lack standardization. Furthermore, clinical breakpoints (CBPs) are not available to interpret AST data (i.e., Minimum Inhibitory Concentration, MIC values) and categorize isolates as susceptible, resistant, or intermediate to the different antimicrobials used in livestock, nor epidemiological cut-offs (ECOFFs), which are a prerequisite to define CBPs. In 2023, the MyMIC network - a consortium of 22 laboratories specializing in veterinary mycoplasmas- was established to support efforts in standardizing diagnostics and AST, including clinical interpretation. Its initial goals were to (i) review routine diagnostic practices in frontline laboratories and examine veterinarians’ prescribing habits and (ii) assess practices for culture, identification and AST used in expert laboratories and how these practices may affect MIC results as collected for five major livestock pathogens (M. bovis, M. gallisepticum, M. synoviae, M. hyopneumoniae and M. hyorhinis).
Keywords (eng)
MycoplasmaDiagnosisAntimicrobial Susceptibility Testing (AST)Minimum Inhibitory Concentration (MIC)LivestockVeterinary Practices
Type (eng)
journal article
Language
[eng]
Persistent identifier
https://phaidra.vetmeduni.ac.at/o:4780
DOI
10.1186/s12917-025-05154-4
Is in series
Title (deu)
BMC Veterinary Research
Volume
21
Issue
1
ISSN
1746-6148
Issued
2025
Number of pages
16
Publication
Springer
Version type (eng)
version of record (published version)
Date issued
2025
Access rights (eng)
open access
License
CC BY 4.0 International
Rights statement (eng)
© The Author(s) 2025