https://phaidra.vetmeduni.ac.at/o:4780 Advancing standardization of diagnostics and antimicrobial susceptibility testing for pathogenic mycoplasmas of livestock origin: insights from the MyMIC network Jaÿ, Maryne Maryne Jaÿ Autio, Tiina Tiina Autio Becker, Claire A.M. Claire A.M. Becker Bokma, Jade Jade Bokma Boland, Cécile Cécile Boland Boyen, Filip Filip Boyen Catania, Salvatore Salvatore Catania Dudek, Katarzyna Katarzyna Dudek Hurri, Emma Emma Hurri Feberwee, Anneke Anneke Feberwee Gyuranecz, Miklós Miklós Gyuranecz Lysnyansky, Inna Inna Lysnyansky Manso-Silván, Lucía Lucía Manso-Silván Palau-Ribes, Franca Möller Franca Möller Palau-Ribes Ramirez, Ana S. Ana S. Ramirez Ridley, Anne Anne Ridley Spergser, Joachim Joachim Spergser 0000-0002-0164-0179 University of Veterinary Medicine Vienna Vaz, Paola K. Paola K. Vaz Wawegama, Nadeeka Nadeeka Wawegama Wiegel, Jeanine Jeanine Wiegel Heuvelink, Annet E. Annet E. Heuvelink Overesch, Gudrun Gudrun Overesch Gautier-Bouchardon, Anne V. Anne V. Gautier-Bouchardon Tardy, Florence Florence Tardy 0000-0003-3968-4801 Klose, Sara M. Sara M. Klose Bottinelli, Marco Marco Bottinelli Springer journal article open access eng Mycoplasma infections pose a significant economic burden and represent a serious health and welfare concern for the livestock sector. Their control often requires repeated antimicrobial treatments. Antimicrobial susceptibility testing (AST) procedures for veterinary mycoplasmas lack standardization. Furthermore, clinical breakpoints (CBPs) are not available to interpret AST data (i.e., Minimum Inhibitory Concentration, MIC values) and categorize isolates as susceptible, resistant, or intermediate to the different antimicrobials used in livestock, nor epidemiological cut-offs (ECOFFs), which are a prerequisite to define CBPs. In 2023, the MyMIC network - a consortium of 22 laboratories specializing in veterinary mycoplasmas- was established to support efforts in standardizing diagnostics and AST, including clinical interpretation. Its initial goals were to (i) review routine diagnostic practices in frontline laboratories and examine veterinarians’ prescribing habits and (ii) assess practices for culture, identification and AST used in expert laboratories and how these practices may affect MIC results as collected for five major livestock pathogens (M. bovis, M. gallisepticum, M. synoviae, M. hyopneumoniae and M. hyorhinis). Mycoplasma Diagnosis Antimicrobial Susceptibility Testing (AST) Minimum Inhibitory Concentration (MIC) Livestock Veterinary Practices http://creativecommons.org/licenses/by/4.0/ https://phaidra.vetmeduni.ac.at/api/object/o:4780/download 10.1186/s12917-025-05154-4 https://phaidra.vetmeduni.ac.at/o:605 application/pdf BMC Veterinary Research issn:1746-6148 VoR BMC Veterinary Research 21 1 1.93 MB 2025