Advancing standardization of diagnostics and antimicrobial susceptibility testing for pathogenic mycoplasmas of livestock origin: insights from the MyMIC network

Maryne Jaÿ Maryne Jaÿ Tiina Autio Tiina Autio Claire A.M. Becker Claire A.M. Becker Jade Bokma Jade Bokma Cécile Boland Cécile Boland Filip Boyen Filip Boyen Salvatore Catania Salvatore Catania Katarzyna Dudek Katarzyna Dudek Emma Hurri Emma Hurri Anneke Feberwee Anneke Feberwee Miklós Gyuranecz Miklós Gyuranecz Inna Lysnyansky Inna Lysnyansky Lucía Manso-Silván Lucía Manso-Silván Franca Möller Palau-Ribes Franca Möller Palau-Ribes Ana S. Ramirez Ana S. Ramirez Anne Ridley Anne Ridley Joachim Spergser Joachim Spergser Paola K. Vaz Paola K. Vaz Nadeeka Wawegama Nadeeka Wawegama Jeanine Wiegel Jeanine Wiegel Annet E. Heuvelink Annet E. Heuvelink Gudrun Overesch Gudrun Overesch Anne V. Gautier-Bouchardon Anne V. Gautier-Bouchardon Florence Tardy Florence Tardy Sara M. Klose Sara M. Klose Marco Bottinelli Marco Bottinelli Advancing standardization of diagnostics and antimicrobial susceptibility testing for pathogenic mycoplasmas of livestock origin: insights from the MyMIC network Springer 2025 Mycoplasma infections pose a significant economic burden and represent a serious health and welfare concern for the livestock sector. Their control often requires repeated antimicrobial treatments. Antimicrobial susceptibility testing (AST) procedures for veterinary mycoplasmas lack standardization. Furthermore, clinical breakpoints (CBPs) are not available to interpret AST data (i.e., Minimum Inhibitory Concentration, MIC values) and categorize isolates as susceptible, resistant, or intermediate to the different antimicrobials used in livestock, nor epidemiological cut-offs (ECOFFs), which are a prerequisite to define CBPs. In 2023, the MyMIC network - a consortium of 22 laboratories specializing in veterinary mycoplasmas- was established to support efforts in standardizing diagnostics and AST, including clinical interpretation. Its initial goals were to (i) review routine diagnostic practices in frontline laboratories and examine veterinarians’ prescribing habits and (ii) assess practices for culture, identification and AST used in expert laboratories and how these practices may affect MIC results as collected for five major livestock pathogens (M. bovis, M. gallisepticum, M. synoviae, M. hyopneumoniae and M. hyorhinis). PDFDocument eng 2026-01-22T14:17:00.353324Z 2025 Mycoplasma Diagnosis Antimicrobial Susceptibility Testing (AST) Minimum Inhibitory Concentration (MIC) Livestock Veterinary Practices 2022635 b application/pdf http://creativecommons.org/licenses/by/4.0/