Immune Responses Induced by Recombinant Membrane Proteins of Mycoplasma agalactiae in Goats

Title (eng)
Immune Responses Induced by Recombinant Membrane Proteins of Mycoplasma agalactiae in Goats
Author
Beatriz Almeida Sampaio
Multidisciplinary Institute in Health, Federal University of Bahia, Rua Hormindo Barros 58, Vitória da Conquista 45029-094, BA, Brazil
Author
Manoel Neres Santos Júnior
Microbiology Department, State University of Santa Cruz, Rod. Jorge Amado, Km a6, Ilhéus 45662-900, BA, Brazil
Author
Bruna Carolina de Brito Guimarães
Microbiology Department, State University of Santa Cruz, Rod. Jorge Amado, Km a6, Ilhéus 45662-900, BA, Brazil
Author
Emilly Stefane Souza Andres
Multidisciplinary Institute in Health, Federal University of Bahia, Rua Hormindo Barros 58, Vitória da Conquista 45029-094, BA, Brazil
Author
Ágatha Morgana Bertoti da Silva
Multidisciplinary Institute in Health, Federal University of Bahia, Rua Hormindo Barros 58, Vitória da Conquista 45029-094, BA, Brazil
Author
Camila Pacheco Gomes
Microbiology Department, State University of Santa Cruz, Rod. Jorge Amado, Km a6, Ilhéus 45662-900, BA, Brazil
Author
Rafaela de Souza Bittencourt
Multidisciplinary Institute in Health, Federal University of Bahia, Rua Hormindo Barros 58, Vitória da Conquista 45029-094, BA, Brazil
Author
Thiago Macêdo Lopes Correia
Multidisciplinary Institute in Health, Federal University of Bahia, Rua Hormindo Barros 58, Vitória da Conquista 45029-094, BA, Brazil
Author
Lucas Santana Coelho da Silva
Microbiology Department, State University of Santa Cruz, Rod. Jorge Amado, Km a6, Ilhéus 45662-900, BA, Brazil
Author
Jurandir Ferreira da Cruz
Goat Sheep Sector, Department of Plant Science and Animal Science, State University of Southwest Bahia, Estrada do Bem Querer, Km 4, Vitória da Conquista 45083-900, BA, Brazil
Author
Rohini Chopra-Dewasthaly
Department of Pathobiology, Institute of Microbiology, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, Austria
Author
Guilherme Barreto Campos
Multidisciplinary Institute in Health, Federal University of Bahia, Rua Hormindo Barros 58, Vitória da Conquista 45029-094, BA, Brazil
Author
Jorge Timenetsky
Institute of Biomedical Sciences, University of São Paulo, Avenida Professor Lineu Prestes, 2415, Butantã 05508-900, SP, Brazil
Author
Bruno Lopes Bastos
Multidisciplinary Institute in Health, Federal University of Bahia, Rua Hormindo Barros 58, Vitória da Conquista 45029-094, BA, Brazil
Author
Lucas Miranda Marques
Multidisciplinary Institute in Health, Federal University of Bahia, Rua Hormindo Barros 58, Vitória da Conquista 45029-094, BA, Brazil
Author
Maysa Santos Barbosa
Institute of Biomedical Sciences, University of São Paulo, Avenida Professor Lineu Prestes, 2415, Butantã 05508-900, SP, Brazil
Author
Matheus Gonçalves de Oliveira
Multidisciplinary Institute in Health, Federal University of Bahia, Rua Hormindo Barros 58, Vitória da Conquista 45029-094, BA, Brazil
Abstract (eng)
Background/Objectives: Contagious agalactia (CA) is a disease typically caused by Mycoplasma agalactiae, affecting small ruminants worldwide and being endemic in certain countries. CA causes severe economic losses due to mastitis, agalactia, and arthritis. As an alternative to existing immunoprophylactic measures, this study aimed to develop a recombinant subunit vaccine against M. agalactiae and evaluate its specific immune response in goats. Methods: Goats were divided into three groups: group 1 received recombinant proteins (P40 and MAG_1560), group 2 received formalin-inactivated M. agalactiae, and group 3 received Tris-buffered saline (negative control). All solutions were emulsified in Freund’s adjuvant. Animals were monitored for 181 days. IgG antibody production was assessed by ELISA, and peripheral blood mononuclear cells (PBMCs) were analyzed by real-time PCR for the expression of IL-1β, IFN-γ, IL-12, and MHC class II genes. Results: M. agalactiae-specific antibody response was observed for six months in the sera of animals from group 1. Analysis of cytokine gene expression revealed increased IL-1β mRNA levels over time in both experimental groups. In group 1, IFN-γ mRNA levels increased with P40 stimulation and decreased with MAG_1560. IL-12 mRNA expression decreased over time in group 1 with P40 stimulation, whereas group 2 showed increased IL-12 expression for both proteins. MHC-II expression was stimulated in both groups. Conclusions: The recombinant proteins induced antibody production and cytokine expression, demonstrating immunogenic potential and supporting their promise as vaccine candidates capable of eliciting both humoral and cellular immune responses against M. agalactiae.
Keywords (eng)
Contagious AgalactiaM. AgalactiaeRecombinant;SubunitVaccine
Type (eng)
journal article
Language
[eng]
Persistent identifier
https://phaidra.vetmeduni.ac.at/o:4915
DOI
10.3390/vaccines13070746
Is in series
Title (eng)
Vaccines
Volume
13
Issue
7
ISSN
2076-393X
Issued
2025
Number of pages
18
Publication
MDPI
Version type (eng)
version of record (published version)
Date issued
2025
Access rights (eng)
open access
License
CC BY 4.0 International
Rights statement (eng)
© 2025 by the authors