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<lom:catalog>phaidra.vetmeduni.ac.at</lom:catalog>

  
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<lom:langstring xml:lang="x-none">o:4915</lom:langstring>

  
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<lom:identifier>
  
<lom:catalog>DOI</lom:catalog>

  
<lom:entry>
  
<lom:langstring xml:lang="x-none">10.3390/vaccines13070746</lom:langstring>

  
</lom:entry>

  
</lom:identifier>

  
<lom:title>
  
<lom:langstring xml:lang="en">Immune Responses Induced by Recombinant Membrane Proteins of Mycoplasma agalactiae in Goats</lom:langstring>

  
</lom:title>

  
<lom:description>
  
<lom:langstring xml:lang="en">Background/Objectives: Contagious agalactia (CA) is a disease typically caused by Mycoplasma agalactiae, affecting small ruminants worldwide and being endemic in certain countries. CA causes severe economic losses due to mastitis, agalactia, and arthritis. As an alternative to existing immunoprophylactic measures, this study aimed to develop a recombinant subunit vaccine against M. agalactiae and evaluate its specific immune response in goats. Methods: Goats were divided into three groups: group 1 received recombinant proteins (P40 and MAG_1560), group 2 received formalin-inactivated M. agalactiae, and group 3 received Tris-buffered saline (negative control). All solutions were emulsified in Freund’s adjuvant. Animals were monitored for 181 days. IgG antibody production was assessed by ELISA, and peripheral blood mononuclear cells (PBMCs) were analyzed by real-time PCR for the expression of IL-1β, IFN-γ, IL-12, and MHC class II genes. Results: M. agalactiae-specific antibody response was observed for six months in the sera of animals from group 1. Analysis of cytokine gene expression revealed increased IL-1β mRNA levels over time in both experimental groups. In group 1, IFN-γ mRNA levels increased with P40 stimulation and decreased with MAG_1560. IL-12 mRNA expression decreased over time in group 1 with P40 stimulation, whereas group 2 showed increased IL-12 expression for both proteins. MHC-II expression was stimulated in both groups. Conclusions: The recombinant proteins induced antibody production and cytokine expression, demonstrating immunogenic potential and supporting their promise as vaccine candidates capable of eliciting both humoral and cellular immune responses against M. agalactiae.</lom:langstring>

  
</lom:description>

  
<lom:language>eng</lom:language>

  
<lom:keyword>
  
<lom:langstring xml:lang="en">Contagious Agalactia</lom:langstring>

  
</lom:keyword>

  
<lom:keyword>
  
<lom:langstring xml:lang="en">M. Agalactiae</lom:langstring>

  
</lom:keyword>

  
<lom:keyword>
  
<lom:langstring xml:lang="en">Recombinant;</lom:langstring>

  
</lom:keyword>

  
<lom:keyword>
  
<lom:langstring xml:lang="en">Subunit</lom:langstring>

  
</lom:keyword>

  
<lom:keyword>
  
<lom:langstring xml:lang="en">Vaccine</lom:langstring>

  
</lom:keyword>

  
</lom:general>

  
<lom:lifecycle>
  
<lom:datetime>2026-02-11T09:28:11.569Z</lom:datetime>

  
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<lom:role>
  
<lom:source>
  
<lom:langstring xml:lang="x-none">LOMv1.0</lom:langstring>

  
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<lom:value>
  
<lom:langstring xml:lang="x-none">Author</lom:langstring>

  
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<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Sampaio;Beatriz Almeida;
FN:Beatriz Almeida Sampaio
X-ORCID:https://orcid.org/0000-0002-2103-5945
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Santos Júnior;Manoel Neres;
FN:Manoel Neres Santos Júnior
X-ORCID:https://orcid.org/0000-0001-8181-4556
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Guimarães;Bruna Carolina de Brito;
FN:Bruna Carolina de Brito Guimarães
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Andres;Emilly Stefane Souza;
FN:Emilly Stefane Souza Andres
X-ORCID:https://orcid.org/0009-0007-2816-3361
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:da Silva;Ágatha Morgana Bertoti;
FN:Ágatha Morgana Bertoti da Silva
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Gomes;Camila Pacheco;
FN:Camila Pacheco Gomes
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Bittencourt;Rafaela de Souza;
FN:Rafaela de Souza Bittencourt
END:VCARD</lom:vcard>

  
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<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Correia;Thiago Macêdo Lopes;
FN:Thiago Macêdo Lopes Correia
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:da Silva;Lucas Santana Coelho;
FN:Lucas Santana Coelho da Silva
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:da Cruz;Jurandir Ferreira;
FN:Jurandir Ferreira da Cruz
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Chopra-Dewasthaly;Rohini;
FN:Rohini Chopra-Dewasthaly
X-ORCID:https://orcid.org/0000-0002-6768-1256
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Campos;Guilherme Barreto;
FN:Guilherme Barreto Campos
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Timenetsky;Jorge;
FN:Jorge Timenetsky
END:VCARD</lom:vcard>

  
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<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Bastos;Bruno Lopes;
FN:Bruno Lopes Bastos
END:VCARD</lom:vcard>

  
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<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Marques;Lucas Miranda;
FN:Lucas Miranda Marques
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Barbosa;Maysa Santos;
FN:Maysa Santos Barbosa
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:de Oliveira;Matheus Gonçalves;
FN:Matheus Gonçalves de Oliveira
END:VCARD</lom:vcard>

  
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<lom:source>
  
<lom:langstring xml:lang="x-none">LOMv1.0</lom:langstring>

  
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<lom:langstring xml:lang="x-none">yes</lom:langstring>

  
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<lom:format>application/pdf</lom:format>

  
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<lom:location>https://phaidra.vetmeduni.ac.at/o:4915</lom:location>

  
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