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Title (eng)
Autophosphorylation of conserved yeast and human casein kinase 1 isozymes regulates Elongator-dependent tRNA modifications
Author
Maria Friederike Landrock
Author
Roscislaw Krutyholowa
Author
Pauline Bohnert
Author
Jaroslaw Mazur
Author
Malgorzata Honc
Author
Alexander Hammermeister
Author
Larissa Bessler
Author
David Scherf
Author
Anna Elms
Author
Natalia Radczuk
Author
Bozena Skupien-Rabian
Author
Urszula Jankowska
Author
Friedrich W. Herberg
Author
Mark Helm
Author
Roland Klassen
Author
Sebastian Glatt
Author
Raffael Schaffrath
Abstract (eng)
Casein kinase 1 (CK1) family members are crucial for ER-Golgi trafficking, calcium signalling, DNA repair, transfer RNA (tRNA) modifications, and circadian rhythmicity. Whether and how substrate interactions and kinase autophosphorylation contribute to CK1 plasticity remains largely unknown. Here, we undertake a comprehensive phylogenetic, cellular, and molecular characterization of budding yeast CK1 Hrr25 and identify human CK1 epsilon (CK1ϵ) as its ortholog. We analyse the effect of Hrr25 depletion and catalytically inactive mutants in vivo and show that perturbations in CK1 activity lead to stress-induced growth defects, morphological abnormalities, and loss of Elongator-dependent tRNA modification. We use purified Hrr25 protein to identify distinct autophosphorylation patterns and phospho-sites on several physiological substrates in vitro and find only human isozyme CK1ϵ can replace yeast Hrr25 functions essential for tRNA modification and cell proliferation in vivo. Furthermore, we demonstrate that human and yeast CK1 orthologs share conserved autophosphorylation sites within the kinase domains, which regulate their activities and mutually exclusive interactions with Elongator subunit Elp1 and Sit4, a phosphatase antagonist of Hrr25. Thus, autophosphorylation controls CK1 activity and regulates the tRNA modification pathway. Our data offer mechanistic insights into regulatory roles of CK1 that are conserved between yeast and human cells and reveal a complex phosphorylation network behind CK1 plasticity.
Type (eng)
journal article
Language
[eng]
Persistent identifier
https://phaidra.vetmeduni.ac.at/o:4485
DOI
10.1093/nar/gkaf881
Is in series
Title (eng)
Nucleic Acids Research
Volume
53
Issue
17
ISSN
1362-4962
Issued
2025
Number of pages
18
Publication
Oxford University Press
Version type (eng)
version of record (published version)
Date issued
2025
Access rights (eng)
open access
License
CC BY 4.0 International
Rights statement (eng)
Copyright © 2025, © The Author(s) 2025.