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<lom:catalog>DOI</lom:catalog>

  
<lom:entry>
  
<lom:langstring xml:lang="x-none">10.1093/nar/gkaf881</lom:langstring>

  
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</lom:identifier>

  
<lom:title>
  
<lom:langstring xml:lang="en">Autophosphorylation of conserved yeast and human casein kinase 1 isozymes regulates Elongator-dependent tRNA modifications</lom:langstring>

  
</lom:title>

  
<lom:description>
  
<lom:langstring xml:lang="en">Casein kinase 1 (CK1) family members are crucial for ER-Golgi trafficking, calcium signalling, DNA repair, transfer RNA (tRNA) modifications, and circadian rhythmicity. Whether and how substrate interactions and kinase autophosphorylation contribute to CK1 plasticity remains largely unknown. Here, we undertake a comprehensive phylogenetic, cellular, and molecular characterization of budding yeast CK1 Hrr25 and identify human CK1 epsilon (CK1ϵ) as its ortholog. We analyse the effect of Hrr25 depletion and catalytically inactive mutants in vivo and show that perturbations in CK1 activity lead to stress-induced growth defects, morphological abnormalities, and loss of Elongator-dependent tRNA modification. We use purified Hrr25 protein to identify distinct autophosphorylation patterns and phospho-sites on several physiological substrates in vitro and find only human isozyme CK1ϵ can replace yeast Hrr25 functions essential for tRNA modification and cell proliferation in vivo. Furthermore, we demonstrate that human and yeast CK1 orthologs share conserved autophosphorylation sites within the kinase domains, which regulate their activities and mutually exclusive interactions with Elongator subunit Elp1 and Sit4, a phosphatase antagonist of Hrr25. Thus, autophosphorylation controls CK1 activity and regulates the tRNA modification pathway. Our data offer mechanistic insights into regulatory roles of CK1 that are conserved between yeast and human cells and reveal a complex phosphorylation network behind CK1 plasticity.</lom:langstring>

  
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<lom:language>eng</lom:language>

  
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<lom:datetime>2025-11-03T13:20:11.851Z</lom:datetime>

  
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<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Landrock;Maria Friederike;
FN:Maria Friederike Landrock
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<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Krutyholowa;Roscislaw;
FN:Roscislaw Krutyholowa
END:VCARD</lom:vcard>

  
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<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Bohnert;Pauline;
FN:Pauline Bohnert
END:VCARD</lom:vcard>

  
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<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Mazur;Jaroslaw;
FN:Jaroslaw Mazur
END:VCARD</lom:vcard>

  
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<lom:vcard>BEGIN:VCARD
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N:Honc;Malgorzata;
FN:Malgorzata Honc
END:VCARD</lom:vcard>

  
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N:Hammermeister;Alexander;
FN:Alexander Hammermeister
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N:Bessler;Larissa;
FN:Larissa Bessler
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<lom:vcard>BEGIN:VCARD
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N:Scherf;David;
FN:David Scherf
END:VCARD</lom:vcard>

  
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<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Elms;Anna;
FN:Anna Elms
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Radczuk;Natalia;
FN:Natalia Radczuk
END:VCARD</lom:vcard>

  
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<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Skupien-Rabian;Bozena;
FN:Bozena Skupien-Rabian
END:VCARD</lom:vcard>

  
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<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Jankowska;Urszula;
FN:Urszula Jankowska
END:VCARD</lom:vcard>

  
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<lom:vcard>BEGIN:VCARD
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N:Herberg;Friedrich W.;
FN:Friedrich W. Herberg
END:VCARD</lom:vcard>

  
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<lom:vcard>BEGIN:VCARD
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N:Helm;Mark;
FN:Mark Helm
END:VCARD</lom:vcard>

  
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<lom:vcard>BEGIN:VCARD
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N:Klassen;Roland;
FN:Roland Klassen
END:VCARD</lom:vcard>

  
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<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Glatt;Sebastian;
FN:Sebastian Glatt
X-ORCID:https://orcid.org/0000-0003-2815-7133
END:VCARD</lom:vcard>

  
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<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Schaffrath;Raffael;
FN:Raffael Schaffrath
END:VCARD</lom:vcard>

  
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