Titel (eng)

Diversity of Staphylococcus aureus associated with mastitis from dairy cows in Rwanda

Autor*in

Helga Keinprecht   University of Veterinary Medicine Vienna / New Vision Veterinary Hospital

Igor Loncaric   University of Veterinary Medicine Vienna

Tom Grunert   University of Veterinary Medicine Vienna

Werner Ruppitsch   Austrian Agency for Health and Food Safety

Ralf Ehricht   Leibniz Institute of Photonic Technology / Friedrich Schiller University Jena

Stefan Monecke   Leibniz Institute of Photonic Technology / Universitätsklinik Dresden

Monika Ehling-Schulz   University of Veterinary Medicine Vienna

Joachim Spergser   University of Veterinary Medicine Vienna

Stefan Schwarz   Freie Universität Berlin

Sascha D. Braun   Leibniz Institute of Photonic Technology / InfectoGnostics Research Campus

Andrea T. Feßler   Freie Universität Berlin

Amelie Desvars-Larrive   University of Veterinary Medicine Vienna / Complexity Science Hub Vienna

Elke Müller   Leibniz Institute of Photonic Technology / InfectoGnostics Research Campus

Thomas Wittek   University of Veterinary Medicine Vienna

Jennifer Zöchbauer   University of Veterinary Medicine Vienna

Michael P. Szostak   University of Veterinary Medicine Vienna

Otto W. Fischer   New Vision Veterinary Hospital

Lydia Marek   University of Veterinary Medicine Vienna / New Vision Veterinary Hospital

Emmanuel Irimaso   University of Rwanda

Adriana Cabal Rosel   Austrian Agency for Health and Food Safety

Beatrix Stessl   University of Veterinary Medicine Vienna

Christophe Ntakirutimana   New Vision Veterinary Hospital

Verlag

Elsevier

Beschreibung (eng)

The objective of the present study was to examine the diversity of Staphylococcus aureus from mastitis milk samples of cows in Rwanda.A total of 1080 quarter milk samples from 279 dairy cows were collected in 80 different farms from all five provinces of Rwanda. In total, 135 S. aureus isolates were obtained and subjected to genotyping (spa typing, DNA microarray, whole-genome sequencing (WGS)), antimicrobial susceptibility testing (AST) and phenotypic profiling by Fourier Transform Infrared (FTIR) spectroscopy (including capsular serotyping).Resistance to penicillin and/or tetracycline was most frequently observed. Ten sequence types (STs) (ST1, ST151, ST152, ST5477, ST700, ST7110, ST7983, ST7984, ST8320, ST97) belonging to seven clonal complexes (CCs) (CC1, CC130, CC152, CC3591, CC3666, CC705, CC97) were detected. The Panton-Valentine leukocidin (PVL) genes (lukF-PV/lukS-PV), the bovine leukocidin genes (lukM/lukF-P83) and the human and bovine toxic shock syndrome toxin gene tst-1 variants were detected. FTIR-based capsular serotyping showed CC-specific differences. Most CC97 (cap5 allele) isolates were primarily nonencapsulated (82%), whereas isolates of CC3591 and CC3666 (cap8 allele) were mostly encapsulated (86.4% and 57.8%, respectively). Our results underline the widespread global distribution of cattle-adapted CC97.The presence of CC3591 and CC3666 in bovine mastitis suggests an important role in cattle health and dairy production in Rwanda. The results of the present study support the need for a rigorous One-Health Surveillance program of the bovine-human interface.

Sprache des Objekts

Englisch

Datum

2024

Rechte

Dieses Werk bzw. dieser Inhalt steht unter einer
CC BY-NC-ND 4.0 - Creative Commons Namensnennung - Nicht-kommerziell - Keine Bearbeitungen 4.0 International Lizenz.

CC BY-NC-ND 4.0 International

http://creativecommons.org/licenses/by-nc-nd/4.0/

Klassifikation

Female; Cattle; Animals; Humans; Staphylococcus aureus; Rwandaepidemiology; Staphylococcal Infectionsepidemiologyveterinary; Anti-Bacterial Agentspharmacology; Mastitis

Mitglied in der/den Collection(s) (1)