Titel (eng)

Investigation of the presence of specific neural antibodies in dogs with epilepsy or dyskinesia using murine and human assays

Autor*in

Lea Hemmeter   LMU Munich

Holger A. Volk   University of Veterinary Medicine Hannover

Kai Rentmeister   Tierärztliche Praxis für Neurologie

Carina Rotter Black   Fitzpatrick Referrals

Clare Rusbridge   University of Surrey

Maik Dahlhoff   University of Veterinary Medicine Vienna

Kaspar Matiasek   LMU Munich

Andrea Bathen-Nöthen   Tierarztpraxis

Jasmin Neßler   University of Veterinary Medicine Hannover

Andrea Tipold   University of Veterinary Medicine Hannover

Christian G. Bien   Bielefeld University

Corinna I. Bien   Laboratory Krone

Verlag

Wiley

Beschreibung (eng)

Autoimmune mechanisms represent a novel category for causes of seizures and epilepsies in humans, and LGI1-antibody associated limbic encephalitis occurs in cats.To investigate the presence of neural antibodies in dogs with epilepsy or dyskinesia of unknown cause using human and murine assays modified for use in dogs.Fifty-eight dogs with epilepsy of unknown cause or suspected dyskinesia and 57 control dogs.Serum and CSF samples were collected prospectively as part of the diagnostic work-up. Clinical data including onset and seizure/episode type were retrieved from the medical records. Screening for neural antibodies was done with cell-based assays transfected with human genes for typical autoimmune encephalitis antigens and tissue-based immunofluorescence assays on mouse hippocampus slices in serum and CSF samples from affected dogs and controls. The commercial human und murine assays were modified with canine-specific secondary antibody. Positive controls were from human samples.The commercial assays used in this study did not provide unequivocal evidence for presence of neural antibodies in dogs including one dog with histopathologically proven limbic encephalitis. Low titer IgLON5 antibodies were present in serum from one dog from the epilepsy/dyskinesia group and in one dog from the control group.Specific neural antibodies were not detected using mouse and human target antigens in dogs with epilepsy and dyskinesia of unknown origin. These findings emphasize the need for canine-specific assays and the importance of control groups.

Sprache des Objekts

Englisch

Datum

2023

Rechte

Dieses Werk bzw. dieser Inhalt steht unter einer
CC BY 4.0 - Creative Commons Namensnennung 4.0 International Lizenz.

CC BY 4.0 International

http://creativecommons.org/licenses/by/4.0/

Klassifikation

Limbic Encephalitis; Clinical Approach; Autoimmune; Autoantibodies; Cats; Diagnosis; Mri; Pet

Mitglied in der/den Collection(s) (1)