Hemmeter, Lea (LMU Munich) Lea Hemmeter Tipold, Andrea (University of Veterinary Medicine Hannover) Andrea Tipold Bien, Christian G. (Bielefeld University) Christian G. Bien Bien, Corinna I. (Laboratory Krone) Corinna I. Bien Volk, Holger A. (University of Veterinary Medicine Hannover) Holger A. Volk Rentmeister, Kai (Tierärztliche Praxis für Neurologie) Kai Rentmeister Rotter Black, Carina (Fitzpatrick Referrals) Carina Rotter Black Rusbridge, Clare (University of Surrey) Clare Rusbridge Dahlhoff, Maik (University of Veterinary Medicine Vienna) Maik Dahlhoff Matiasek, Kaspar (LMU Munich) Kaspar Matiasek Bathen-Nöthen, Andrea (Tierarztpraxis) Andrea Bathen-Nöthen Neßler, Jasmin (University of Veterinary Medicine Hannover) Jasmin Neßler Wiley 2023 Autoimmune mechanisms represent a novel category for causes of seizures and epilepsies in humans, and LGI1-antibody associated limbic encephalitis occurs in cats.To investigate the presence of neural antibodies in dogs with epilepsy or dyskinesia of unknown cause using human and murine assays modified for use in dogs.Fifty-eight dogs with epilepsy of unknown cause or suspected dyskinesia and 57 control dogs.Serum and CSF samples were collected prospectively as part of the diagnostic work-up. Clinical data including onset and seizure/episode type were retrieved from the medical records. Screening for neural antibodies was done with cell-based assays transfected with human genes for typical autoimmune encephalitis antigens and tissue-based immunofluorescence assays on mouse hippocampus slices in serum and CSF samples from affected dogs and controls. The commercial human und murine assays were modified with canine-specific secondary antibody. Positive controls were from human samples.The commercial assays used in this study did not provide unequivocal evidence for presence of neural antibodies in dogs including one dog with histopathologically proven limbic encephalitis. Low titer IgLON5 antibodies were present in serum from one dog from the epilepsy/dyskinesia group and in one dog from the control group.Specific neural antibodies were not detected using mouse and human target antigens in dogs with epilepsy and dyskinesia of unknown origin. These findings emphasize the need for canine-specific assays and the importance of control groups. PDFDocument en 2023-11-09T13:57:08.921Z Limbic Encephalitis; Clinical Approach; Autoimmune; Autoantibodies; Cats; Diagnosis; Mri; Pet 867146 b application/pdf CC BY 4.0 International