Footprints of Worldwide Adaptation in Structured Populations of "Drosophila melanogaster" Through the Expanded DEST 2.0 Genomic Resource

Title (eng)
Footprints of Worldwide Adaptation in Structured Populations of "Drosophila melanogaster" Through the Expanded DEST 2.0 Genomic Resource
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Antonio J Buendia-Ruíz
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Bélen Roldán Matrín
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Christian Feller
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Christian Schlötterer
Genetics and Animal Breeding, Centre of Biological Sciences, Department of Biological Sciences and Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria
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Joan Torro
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Jose Luis Olmo
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Krzysztof Wojciechowski
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M Josefa Gómez-Julián
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M Luisa Espinosa-Jimenez
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Paloma Sepulveda
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Jorge Roberto Torres
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Silvana Castillo
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Zahara Alonso
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Abstract (eng)
Large-scale genomic resources can place genetic variation into an ecologically informed context. To advance our understanding of the population genetics of the fruit fly Drosophila melanogaster, we present an expanded release of the community-generated population genomics resource Drosophila Evolution over Space and Time (DEST 2.0; dest.bio). This release includes 530 high-quality pooled libraries from flies collected across six continents over more than a decade (2009 to 2021), most at multiple time points per year; 211 of these libraries are sequenced and shared here for the first time. We used this enhanced resource to elucidate several aspects of the species' demographic history and identify novel signs of adaptation across spatial and temporal dimensions. For example, we showed that the spatial genetic structure of populations is stable over time, but that drift due to seasonal contractions of population size causes populations to diverge over time. We identified signals of adaptation that vary between continents in genomic regions associated with xenobiotic resistance, consistent with independent adaptation to common pesticides. Moreover, by analyzing samples collected during spring and fall across Europe, we provide new evidence for seasonal adaptation related to loci associated with pathogen response. Furthermore, we have also released an updated version of the DEST genome browser. This is a useful tool for studying spatiotemporal patterns of genetic variation in this classic model system.
Keywords (eng)
Drosophila MelanogasterDatasetPopulation StructureSeasonal SelectionLocal AdaptionEcological Genomics
Type (eng)
Language
[eng]
Is in series
Title (eng)
Molecular Biology and Evolution
Volume
42
Issue
8
ISSN
0737-4038
Issued
2025
Number of pages
28
Publication
Oxford University Press
Date issued
2025
Access rights (eng)
Rights statement (eng)
© The Author(s) 2025