Joaquin C B Nunez Joaquin C B Nunez Marta Coronado-Zamora Marta Coronado-Zamora Mathieu Gautier Mathieu Gautier Martin Kapun Martin Kapun Sonja Steindl Sonja Steindl Lino Ometto Lino Ometto Katja Hoedjes Katja Hoedjes Julia Beets Julia Beets R Axel W Wiberg R Axel W Wiberg Giovanni R Mazzeo Giovanni R Mazzeo David J Bass David J Bass Denys Radionov Denys Radionov Iryna Kozeretska Iryna Kozeretska Mariia Zinchenko Mariia Zinchenko Oleksandra Protsenko Oleksandra Protsenko Svitlana V Serga Svitlana V Serga Cristina Amor-Jimenez Cristina Amor-Jimenez Sònia Casillas Sònia Casillas Alejandro Sánchez-Gracia Alejandro Sánchez-Gracia Aleksandra Patenkovic Aleksandra Patenkovic Amanda Glaser-Schmitt Amanda Glaser-Schmitt Antonio Barbadilla Antonio Barbadilla Antonio J Buendia-Ruíz Antonio J Buendia-Ruíz Astra Clelia Bertelli Astra Clelia Bertelli Balázs Kiss Balázs Kiss Banu Sebnem Önder Banu Sebnem Önder Bélen Roldán Matrín Bélen Roldán Matrín Bregje Wertheim Bregje Wertheim Candice Deschamps Candice Deschamps Carlos E Arboleda-Bustos Carlos E Arboleda-Bustos Carlos Tinedo Carlos Tinedo Christian Feller Christian Feller Christian Schlötterer (Genetics and Animal Breeding, Centre of Biological Sciences, Department of Biological Sciences and Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria) Christian Schlötterer Clancy Lawler Clancy Lawler Claudia Fricke Claudia Fricke Cristina P Vieira Cristina P Vieira Cristina Vieira Cristina Vieira Darren J Obbard Darren J Obbard Dorcas Juana Orengo Dorcas Juana Orengo Doris Vela Doris Vela Eduardo Amat Eduardo Amat Elgion Loreto Elgion Loreto Envel Kerdaffrec Envel Kerdaffrec Esra Durmaz Mitchell Esra Durmaz Mitchell Eva Puerma Eva Puerma Fabian Staubach Fabian Staubach M Florencia Camus M Florencia Camus Hervé Colinet Hervé Colinet Jan Hrcek Jan Hrcek Jesper Givskov Sørensen Jesper Givskov Sørensen Jessica Abbott Jessica Abbott Joan Torro Joan Torro John Parsch John Parsch Jorge Vieira Jorge Vieira Jose Luis Olmo Jose Luis Olmo Khalid Khfif Khalid Khfif Krzysztof Wojciechowski Krzysztof Wojciechowski Lilian Madi-Ravazzi Lilian Madi-Ravazzi Maaria Kankare Maaria Kankare Mads F Schou Mads F Schou Emmanuel D Ladoukakis Emmanuel D Ladoukakis M Josefa Gómez-Julián M Josefa Gómez-Julián M Luisa Espinosa-Jimenez M Luisa Espinosa-Jimenez Maria Pilar Garcia Guerreiro Maria Pilar Garcia Guerreiro Maria-Eleni Parakatselaki Maria-Eleni Parakatselaki Marija Savic Veselinovic Marija Savic Veselinovic Marija Tanaskovic Marija Tanaskovic Marina Stamenkovic-Radak Marina Stamenkovic-Radak Margot Paris Margot Paris Marta Pascual Marta Pascual Michael G Ritchie Michael G Ritchie Michel Rera Michel Rera Mihailo Jelić Mihailo Jelić Mina Hojat Ansari Mina Hojat Ansari Mina Rakic Mina Rakic Miriam Merenciano Miriam Merenciano Natalia Hernandes Natalia Hernandes Nazar Gora Nazar Gora Nicolas Rode Nicolas Rode Omar Rota-Stabelli Omar Rota-Stabelli Paloma Sepulveda Paloma Sepulveda Patricia Gibert Patricia Gibert Pau Carazo Pau Carazo Pinar Kohlmeier Pinar Kohlmeier Priscilla A Erickson Priscilla A Erickson Renaud Vitalis Renaud Vitalis Jorge Roberto Torres Jorge Roberto Torres Sara Guirao-Rico Sara Guirao-Rico Sebastian E Ramos-Onsins Sebastian E Ramos-Onsins Silvana Castillo Silvana Castillo Tânia F Paulo Tânia F Paulo Venera Tyukmaeva Venera Tyukmaeva Zahara Alonso Zahara Alonso Vladimir E Alatortsev Vladimir E Alatortsev Elena Pasyukova Elena Pasyukova Dmitry V Mukha Dmitry V Mukha Dmitri A Petrov Dmitri A Petrov Paul Schmidt Paul Schmidt Thomas Flatt Thomas Flatt Alan O Bergland Alan O Bergland Josefa Gonzalez Josefa Gonzalez Oxford University Press 2025 Large-scale genomic resources can place genetic variation into an ecologically informed context. To advance our understanding of the population genetics of the fruit fly Drosophila melanogaster, we present an expanded release of the community-generated population genomics resource Drosophila Evolution over Space and Time (DEST 2.0; https://dest.bio/). This release includes 530 high-quality pooled libraries from flies collected across six continents over more than a decade (2009 to 2021), most at multiple time points per year; 211 of these libraries are sequenced and shared here for the first time. We used this enhanced resource to elucidate several aspects of the species' demographic history and identify novel signs of adaptation across spatial and temporal dimensions. For example, we showed that the spatial genetic structure of populations is stable over time, but that drift due to seasonal contractions of population size causes populations to diverge over time. We identified signals of adaptation that vary between continents in genomic regions associated with xenobiotic resistance, consistent with independent adaptation to common pesticides. Moreover, by analyzing samples collected during spring and fall across Europe, we provide new evidence for seasonal adaptation related to loci associated with pathogen response. Furthermore, we have also released an updated version of the DEST genome browser. This is a useful tool for studying spatiotemporal patterns of genetic variation in this classic model system. PDFDocument eng 2026-04-20T09:28:19.169268Z 2025 Drosophila Melanogaster Dataset Population Structure Seasonal Selection Local Adaption Ecological Genomics 11446658 b application/pdf http://creativecommons.org/licenses/by-nc/4.0/