https://phaidra.vetmeduni.ac.at/o:5054 Footprints of Worldwide Adaptation in Structured Populations of "Drosophila melanogaster" Through the Expanded DEST 2.0 Genomic Resource Nunez, Joaquin C B Joaquin C B Nunez 0000-0002-3171-8918 Coronado-Zamora, Marta Marta Coronado-Zamora 0000-0001-9779-9661 Gautier, Mathieu Mathieu Gautier 0000-0001-7257-5880 Kapun, Martin Martin Kapun 0000-0002-3810-0504 Steindl, Sonja Sonja Steindl 0009-0007-5354-1950 Ometto, Lino Lino Ometto 0000-0002-2679-625X Hoedjes, Katja Katja Hoedjes 0000-0001-6090-232X Beets, Julia Julia Beets 0009-0005-8375-9884 Wiberg, R Axel W R Axel W Wiberg 0000-0002-8074-8670 Mazzeo, Giovanni R Giovanni R Mazzeo 0009-0008-0898-2041 Bass, David J David J Bass 0000-0001-7414-2747 Radionov, Denys Denys Radionov 0000-0003-0138-1303 Kozeretska, Iryna Iryna Kozeretska 0000-0002-6485-1408 Zinchenko, Mariia Mariia Zinchenko 0000-0001-6485-3668 Protsenko, Oleksandra Oleksandra Protsenko 0000-0002-6019-9731 Serga, Svitlana V Svitlana V Serga 0000-0003-1875-3185 Amor-Jimenez, Cristina Cristina Amor-Jimenez 0009-0009-7822-8199 Casillas, Sònia Sònia Casillas 0000-0001-8191-0062 Sánchez-Gracia, Alejandro Alejandro Sánchez-Gracia 0000-0003-4543-4577 Patenkovic, Aleksandra Aleksandra Patenkovic 0000-0001-5763-6294 Glaser-Schmitt, Amanda Amanda Glaser-Schmitt 0000-0002-1322-1000 Barbadilla, Antonio Antonio Barbadilla 0000-0002-0374-1475 Buendia-Ruíz, Antonio J Antonio J Buendia-Ruíz Bertelli, Astra Clelia Astra Clelia Bertelli 0009-0002-3595-0498 Kiss, Balázs Balázs Kiss 0000-0003-2511-9094 Önder, Banu Sebnem Banu Sebnem Önder 0000-0002-3003-248X Matrín, Bélen Roldán Bélen Roldán Matrín Wertheim, Bregje Bregje Wertheim 0000-0001-8555-1925 Deschamps, Candice Candice Deschamps 0000-0002-5167-5433 Arboleda-Bustos, Carlos E Carlos E Arboleda-Bustos 0000-0002-1783-3063 Tinedo, Carlos Carlos Tinedo 0009-0004-8038-9817 Feller, Christian Christian Feller Schlötterer, Christian Christian Schlötterer 0000-0003-4710-6526 Genetics and Animal Breeding, Centre of Biological Sciences, Department of Biological Sciences and Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria Lawler, Clancy Clancy Lawler 0009-0007-1867-4969 Fricke, Claudia Claudia Fricke 0000-0002-0691-6779 Vieira, Cristina P Cristina P Vieira 0000-0002-7139-2107 Vieira, Cristina Cristina Vieira 0000-0003-3414-3993 Obbard, Darren J Darren J Obbard 0000-0001-5392-8142 Orengo, Dorcas Juana Dorcas Juana Orengo 0000-0001-7911-3224 Vela, Doris Doris Vela 0000-0001-7690-7758 Amat, Eduardo Eduardo Amat 0000-0002-0962-777X Loreto, Elgion Elgion Loreto 0000-0002-7586-8168 Kerdaffrec, Envel Envel Kerdaffrec 0000-0001-8667-6850 Mitchell, Esra Durmaz Esra Durmaz Mitchell 0000-0002-4345-2264 Puerma, Eva Eva Puerma 0000-0001-7261-187X Staubach, Fabian Fabian Staubach 0000-0002-8097-2349 Camus, M Florencia M Florencia Camus 0000-0003-0626-6865 Colinet, Hervé Hervé Colinet 0000-0002-8806-3107 Hrcek, Jan Jan Hrcek 0000-0003-0711-6447 Sørensen, Jesper Givskov Jesper Givskov Sørensen 0000-0002-9149-3626 Abbott, Jessica Jessica Abbott 0000-0002-8743-2089 Torro, Joan Joan Torro Parsch, John John Parsch 0000-0001-9068-5549 Vieira, Jorge Jorge Vieira 0000-0001-7032-5220 Olmo, Jose Luis Jose Luis Olmo Khfif, Khalid Khalid Khfif 0000-0002-3874-8845 Wojciechowski, Krzysztof Krzysztof Wojciechowski Madi-Ravazzi, Lilian Lilian Madi-Ravazzi 0000-0002-6250-0794 Kankare, Maaria Maaria Kankare 0000-0003-1541-9050 Schou, Mads F Mads F Schou 0000-0001-5521-5269 Ladoukakis, Emmanuel D Emmanuel D Ladoukakis 0000-0002-1123-5396 Gómez-Julián, M Josefa M Josefa Gómez-Julián Espinosa-Jimenez, M Luisa M Luisa Espinosa-Jimenez Garcia Guerreiro, Maria Pilar Maria Pilar Garcia Guerreiro 0000-0001-9951-1879 Parakatselaki, Maria-Eleni Maria-Eleni Parakatselaki 0000-0003-0150-6153 Savic Veselinovic, Marija Marija Savic Veselinovic 0000-0001-8461-4373 Tanaskovic, Marija Marija Tanaskovic 0000-0003-1440-2257 Stamenkovic-Radak, Marina Marina Stamenkovic-Radak 0000-0002-6937-7282 Paris, Margot Margot Paris 0000-0001-7328-3820 Pascual, Marta Marta Pascual 0000-0002-6189-0612 Ritchie, Michael G Michael G Ritchie 0000-0001-7913-8675 Rera, Michel Michel Rera 0000-0002-6574-6511 Jelić, Mihailo Mihailo Jelić 0000-0002-1637-0933 Ansari, Mina Hojat Mina Hojat Ansari 0000-0002-3602-7884 Rakic, Mina Mina Rakic 0000-0002-6069-3369 Merenciano, Miriam Miriam Merenciano 0000-0001-8592-949X Hernandes, Natalia Natalia Hernandes 0000-0001-5644-6974 Gora, Nazar Nazar Gora 0000-0003-0589-8799 Rode, Nicolas Nicolas Rode 0000-0002-1121-4202 Rota-Stabelli, Omar Omar Rota-Stabelli 0000-0002-0030-7788 Sepulveda, Paloma Paloma Sepulveda Gibert, Patricia Patricia Gibert 0000-0002-9461-6820 Carazo, Pau Pau Carazo 0000-0002-1525-6522 Kohlmeier, Pinar Pinar Kohlmeier 0009-0001-2404-3625 Erickson, Priscilla A Priscilla A Erickson 0000-0001-8420-995X Vitalis, Renaud Renaud Vitalis 0000-0001-7096-3089 Torres, Jorge Roberto Jorge Roberto Torres Guirao-Rico, Sara Sara Guirao-Rico 0000-0001-9896-4665 Ramos-Onsins, Sebastian E Sebastian E Ramos-Onsins 0000-0002-1776-140X Castillo, Silvana Silvana Castillo Paulo, Tânia F Tânia F Paulo 0000-0002-8815-2636 Tyukmaeva, Venera Venera Tyukmaeva 0000-0002-1864-5101 Alonso, Zahara Zahara Alonso Alatortsev, Vladimir E Vladimir E Alatortsev 0000-0003-4713-6610 Pasyukova, Elena Elena Pasyukova 0000-0002-6491-8561 Mukha, Dmitry V Dmitry V Mukha 0000-0002-7380-0776 Petrov, Dmitri A Dmitri A Petrov 0000-0002-3664-9130 Schmidt, Paul Paul Schmidt 0000-0002-8076-6705 Flatt, Thomas Thomas Flatt 0000-0002-5990-1503 Bergland, Alan O Alan O Bergland 0000-0001-7145-7575 Gonzalez, Josefa Josefa Gonzalez 0000-0001-9824-027X Oxford University Press journal article open access eng Large-scale genomic resources can place genetic variation into an ecologically informed context. To advance our understanding of the population genetics of the fruit fly Drosophila melanogaster, we present an expanded release of the community-generated population genomics resource Drosophila Evolution over Space and Time (DEST 2.0; https://dest.bio/). This release includes 530 high-quality pooled libraries from flies collected across six continents over more than a decade (2009 to 2021), most at multiple time points per year; 211 of these libraries are sequenced and shared here for the first time. We used this enhanced resource to elucidate several aspects of the species' demographic history and identify novel signs of adaptation across spatial and temporal dimensions. For example, we showed that the spatial genetic structure of populations is stable over time, but that drift due to seasonal contractions of population size causes populations to diverge over time. We identified signals of adaptation that vary between continents in genomic regions associated with xenobiotic resistance, consistent with independent adaptation to common pesticides. Moreover, by analyzing samples collected during spring and fall across Europe, we provide new evidence for seasonal adaptation related to loci associated with pathogen response. Furthermore, we have also released an updated version of the DEST genome browser. This is a useful tool for studying spatiotemporal patterns of genetic variation in this classic model system. Drosophila Melanogaster Dataset Population Structure Seasonal Selection Local Adaption Ecological Genomics http://creativecommons.org/licenses/by-nc/4.0/ https://phaidra.vetmeduni.ac.at/api/object/o:5054/download 10.1093/molbev/msaf132 https://phaidra.vetmeduni.ac.at/o:605 application/pdf Molecular Biology and Evolution issn:0737-4038 VoR Molecular Biology and Evolution 42 8 10.92 MB 2025