Pluck-pools as diagnostic samples for detecting porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 in porcine abortion material and stillbirths

Heinrich Kreutzmann; Christine Unterweger; Lukas Schwarz; Katharina Dimmel; Angelika Auer; Till Rümenapf; Andrea Ladinig

doi:10.1016/j.tvjl.2024.106081

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Title (eng)

Pluck-pools as diagnostic samples for detecting porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 in porcine abortion material and stillbirths

Author

Heinrich Kreutzmann

University of Veterinary Medicine Vienna

Christine Unterweger

University of Veterinary Medicine Vienna

Lukas Schwarz

University of Veterinary Medicine Vienna

Katharina Dimmel

University of Veterinary Medicine Vienna

Angelika Auer

University of Veterinary Medicine Vienna

Till Rümenapf

University of Veterinary Medicine Vienna

Andrea Ladinig

University of Veterinary Medicine Vienna

Abstract (eng)

Investigating infectious agents in porcine abortion material and stillborn piglets poses challenges for practitioners and diagnostic laboratories. In this study, pooled samples of individual reference organs (thymus and heart) from a total of 1000 aborted fetuses and stillborn piglets were investigated using quantitative PCR protocols for porcine reproductive and respiratory syndrome virus 1 (PRRSV-1) and porcine circovirus type 2 (PCV2). Simultaneously, a pluck-pool containing equivalent portions of fetal thymus, heart, and lung tissue was collected, frozen at - 20 °C, and re-analyzed when a certain amount of either PRRSV-1 RNA or PCV2 DNA was detected in individual reference organs. Thirteen pluck-pools were assessed for PRRSV-1, all being PCR-positive. For PCV2, 11 of 15 pluck-pools investigated were PCR-positive. In all pluck-pools testing negative, viral loads in individual pools were low. This study indicates that pluck-pools can be valuable diagnostic material and the consolidation of multiple organs through a single RNA/DNA extraction optimizes the utilization of available laboratory resources. Additional research is required to assess the feasibility of follow-up investigations and to accurately define criteria for interpretation of viral loads in a clinical context.

Keywords (eng)

Pluck-PoolPorcine Circovirus Type 2Porcine FetusPorcine Reproductive And Respiratory Syndrome VirusReproductive Failure

Type (eng)

journal article

Language

[eng]

Persistent identifier

https://phaidra.vetmeduni.ac.at/o:4458

DOI

10.1016/j.tvjl.2024.106081

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Title (eng)