Integrated microbiota-host-metabolome approaches reveal adaptive ruminal changes to prolonged high-grain feeding and phytogenic supplementation in cattle.

Sara Ricci; Cátia Pacífico; Susanne Kreuzer-Redmer; Ezequias Castillo-Lopez; Raul Rivera-Chacon; Arife Sener-Aydemir; Giacomo Rossi; Livio Galosi; Lucia Biagini; Heidi E Schwartz-Zimmermann; Franz Berthiller; Nicole Reisinger; Renee M Petri; Qendrim Zebeli

doi:10.1093/femsec/fiae006

You are here:

PHAIDRA
Detail o:4447

Title (eng)

Integrated microbiota-host-metabolome approaches reveal adaptive ruminal changes to prolonged high-grain feeding and phytogenic supplementation in cattle.

Author

Sara Ricci

University of Veterinary Medicine Vienna

Cátia Pacífico

University of Veterinary Medicine Vienna

Susanne Kreuzer-Redmer

University of Veterinary Medicine Vienna

Ezequias Castillo-Lopez

University of Veterinary Medicine Vienna

Raul Rivera-Chacon

University of Veterinary Medicine Vienna

Arife Sener-Aydemir

University of Veterinary Medicine Vienna

Giacomo Rossi

Livio Galosi

Lucia Biagini

Heidi E Schwartz-Zimmermann

Franz Berthiller

Nicole Reisinger

Renee M Petri

Qendrim Zebeli

Abstract (eng)

Diets rich in readily fermentable carbohydrates primarily impact microbial composition and activity, but can also impair the ruminal epithelium barrier function. By combining microbiota, metabolome, and gene expression analysis, we evaluated the impact of feeding a 65% concentrate diet for 4 weeks, with or without a phytogenic feed additive (PFA), on the rumen ecosystem of cattle. The breaking point for rumen health seemed to be the second week of high grain (HG) diet, with a dysbiosis characterized by reduced alpha diversity. While we did not find changes in histological evaluations, genes related with epithelial proliferation (IGF-1, IGF-1R, EGFR, and TBP) and ZO-1 were affected by the HG feeding. Integrative analyses allowed us to define the main drivers of difference for the rumen ecosystem in response to a HG diet, identified as ZO-1, MyD88, and genus Prevotella 1. PFA supplementation reduced the concentration of potentially harmful compounds in the rumen (e.g. dopamine and 5-aminovaleric acid) and increased the tolerance of the epithelium toward the microbiota by altering the expression of TLR-2, IL-6, and IL-10. The particle-associated rumen liquid microbiota showed a quicker adaptation potential to prolonged HG feeding compared to the other microenvironments investigated, especially by the end of the experiment.

Keywords (eng)

BovineCross-TalkGut HealthInflammationMucosal Barrier FunctionPhytogenic Feed Additives

Type (eng)

journal article

Language

[eng]

Persistent identifier

https://phaidra.vetmeduni.ac.at/o:4447

DOI

10.1093/femsec/fiae006

Is in series

Title (eng)