Unveiling the genetic landscape of infectious laryngotracheitis virus in Switzerland: Evidence for vaccine-like and wild-type strains

Sonja Hermann; marc J. A. Stevens; Brigitte Sigrist; Ivana Bilic; Thora Lieke; Sarah Albini; Nina Wolfrum

doi:10.1016/j.virol.2024.110217

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Title (eng)

Unveiling the genetic landscape of infectious laryngotracheitis virus in Switzerland: Evidence for vaccine-like and wild-type strains

Author

Sonja Hermann

marc J. A. Stevens

Brigitte Sigrist

Ivana Bilic

University of Veterinary Medicine Vienna

Thora Lieke

Sarah Albini

Nina Wolfrum

Abstract (eng)

Infectious laryngotracheitis (ILT) is a respiratory disease affecting chickens worldwide. Unlike many countries, Switzerland does not vaccinate against ILT. This study analysed ILT samples from 21 natural outbreaks in Switzerland using restriction fragment length polymorphism (RFLP) and multiple gene sequencing. Chicken embryo origin (CEO) and tissue culture origin (TCO) vaccine strains were included as references. Both vaccine strains were distinguishable, and 14 out of 21 samples resembled the CEO vaccine. Additionally, four distinct non-vaccine-like groups were identified. Sequencing of three genes from selected Swiss samples and those from neighbouring countries revealed four phylogenetic clades. Notably, four Swiss field strains formed two unique clades, not closely related to vaccine strains or ILTV from neighbouring countries. Overall, RFLP results were supported by sequencing data. This study demonstrates the presence of both vaccine-like and wild-type ILT viruses in Switzerland, where vaccination is de facto prohibited.Copyright © 2024 Elsevier Inc. All rights reserved.

Keywords (eng)

Switzerland EpidemiologyAnimalsHerpesvirus 1, Gallid GeneticsHerpesvirus 1, Gallid ClassificationHerpesvirus 1, Gallid ImmunologyHerpesvirus 1, Gallid Isolation & PurificationChickens VirologyPoultry Diseases VirologyPoultry Diseases Prevention & ControlHerpesviridae Infections VeterinaryHerpesviridae Infections VirologyHerpesviridae Infections Prevention & ControlHerpesviridae Infections EpidemiologyPhylogenyPolymorphism, Restriction Fragment LengthViral Vaccines ImmunologyViral Vaccines GeneticsDisease Outbreaks Veterinary

Type (eng)

journal article

Language

[eng]

Persistent identifier

https://phaidra.vetmeduni.ac.at/o:4259

DOI

10.1016/j.virol.2024.110217

Is in series

Title (eng)

Virology

Volume