Title (eng)
Establishing Reference Genes for Accurate Gene Expression Profiling in Toxigenic Bacillus cereus
Abstract (eng)
Bacillus cereus is a Gram-positive pathogen associated with foodborne illnesses and severe non-gastrointestinal infections. Robust tools for accurate gene transcription analysis are essential for studying toxin gene expression dynamics and deciphering the complex regulatory networks orchestrating the expression of toxin and virulence factors. This study aimed to identify reliable reference genes for normalizing reverse transcription quantitative PCR (RT-qPCR) data in toxigenic B. cereus. An emetic and an enteropathogenic strain were used as model organisms to establish a suitable reference gene set to monitor the dynamics of toxin gene transcription. Ten candidate reference genes were evaluated for their expression stability using geNorm, NormFinder, BestKeeper and the ?Cq method, with the final rankings integrated via RefFinder. Among the tested genes, rho, rpoD and recA were identified as the most stable expressed reference genes across all tested conditions. As shown in this proof-of-principle study, the established reference gene set provides a suitable tool to investigate the influence of extrinsic and intrinsic factors on toxin gene transcription. In conclusion, our newly established reference gene set provides a robust basis for studying toxin gene expression in B. cereus and contributes to a better understanding of its pathogenicity and potential strategies to mitigate its harmful effects.
Keywords (eng)
Bacillus Cereus GeneticsBacillus Cereus MetabolismGene Expression Profiling StandardsBacterial Toxins GeneticsGene Expression Regulation, BacterialBacterial Proteins GeneticsReference StandardsGenes, BacterialReal-Time Polymerase Chain Reaction Standards
Type (eng)
Language
[eng]
Persistent identifier
Is in series
Title (eng)
Toxins
Volume
17
Issue
2
ISSN
2072-6651
Issued
2025
Number of pages
17
Publication
MDPI
Version type (eng)
Date issued
2025
Access rights (eng)
License
Rights statement (eng)
© 2025 by the authors
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DOI
https://phaidra.vetmeduni.ac.at/o:3998
https://doi.org/10.3390/toxins17020058 - Content
- DetailsObject typePDFDocumentFormatapplication/pdfCreated02.04.2025 08:47:19 UTC
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