Molecular characterization, virulence and antimicrobial and biocidal susceptibility of selected bacteria isolated from the cloaca of nestling ospreys (Pandion haliaetus) from Mono Lake, California, USA

Igor Loncaric; Michael P. Szostak; Adriana Cabal-Rosel; Olivia M. Grünzweil; Alina Riegelnegg; Dusan Misic; Elke Müller; Andrea T. Feßler; Sascha D. Braun; Stefan Schwarz; Stefan Monecke; Ralf Ehricht; Werner Ruppitsch; Joachim Spergser; Ashli Lewis; Peter H. Bloom; Miguel D. Saggese

doi:10.1371/journal.pone.0311306

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Title (eng)

Molecular characterization, virulence and antimicrobial and biocidal susceptibility of selected bacteria isolated from the cloaca of nestling ospreys (Pandion haliaetus) from Mono Lake, California, USA

Author

Igor Loncaric

Michael P. Szostak

Adriana Cabal-Rosel

Olivia M. Grünzweil

Alina Riegelnegg

Dusan Misic

Elke Müller

Andrea T. Feßler

Sascha D. Braun

Stefan Schwarz

Stefan Monecke

Ralf Ehricht

Werner Ruppitsch

Joachim Spergser

Ashli Lewis

Peter H. Bloom

Miguel D. Saggese

Abstract (eng)

In the present study, the presence of the Enterobacterales, Staphylococcus spp., Mammaliicoccus spp., and Enterococcus spp. in cloacal samples of nestling ospreys (Pandion haliaetus), a fish-eating specialist, from Mono Lake, California, USA was examined by a multiphasic approach, including antimicrobial and biocide susceptibility testing, genotyping, and whole genome sequencing of selected isolates. The most commonly detected species was Escherichia coli, followed by Mammaliicoccus sciuri, Staphylococcus delphini, Enterococcus faecalis, Enterococcus faecium, Hafnia alvei, Klebsiella pneumoniae, Citrobacter braakii and single isolates of Edwardsiella tarda, Edwardsiella albertii, Klebsiella aerogenes, Plesiomonas shigelloides and Staphylococcus pseudintermedius. Multi-drug resistance (MDR) was observed in two E. coli isolates and in an Enterococcus faecium isolate. The MDR blaCTX-M-55-positive E. coli belonged to the pandemic clone ST58. The results of the present study suggest that nestling ospreys are exposed to MDR bacteria, possibly through the ingestion of contaminated fish. Ospreys may be good biosentinels for the presence of these microorganisms and antibiotic resistance in the local environment and the risk for other wildlife, livestock and humans.

Keywords (eng)

AnimalsCaliforniaLakes MicrobiologyCloaca MicrobiologyMicrobial Sensitivity TestsAnti-Bacterial Agents PharmacologyBacteria Isolation & PurificationBacteria Drug EffectsBacteria GeneticsVirulenceDrug Resistance Multiple Bacterial Genetics

Type (eng)

journal article

Language

[eng]

Persistent identifier

https://phaidra.vetmeduni.ac.at/o:3792

DOI

10.1371/journal.pone.0311306

Is in series

Title (eng)

PLOS One

Volume