Survey in ruminants from Rwanda revealed high diversity and prevalence of extended-spectrum cephalosporin-resistant Enterobacterales

Emmanuel Irimaso; Helga Keinprecht; Michael P. Szostak; Adriana Cabal Rosel; Beatrix Stessl; Amelie Desvars-Larrive; Christophe Ntakirutimana; Otto W. Fischer; Thomas Wittek; Elke Müller; Andrea T. Feßler; Sascha D. Braun; Stefan Schwarz; Stefan Monecke; Ralf Ehricht; Joachim Spergser; Werner Ruppitsch; Igor Loncaric

doi:10.1186/s12917-024-04359-3

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Title (eng)

Survey in ruminants from Rwanda revealed high diversity and prevalence of extended-spectrum cephalosporin-resistant Enterobacterales

Author

Emmanuel Irimaso

Helga Keinprecht

Michael P. Szostak

Adriana Cabal Rosel

Beatrix Stessl

Amelie Desvars-Larrive

Christophe Ntakirutimana

Otto W. Fischer

Thomas Wittek

Elke Müller

Andrea T. Feßler

Sascha D. Braun

Stefan Schwarz

Stefan Monecke

Ralf Ehricht

Joachim Spergser

Werner Ruppitsch

Igor Loncaric

Abstract (eng)

Antimicrobial resistance (AMR) in Enterobacterales constitutes a significant threat to the health of both humans and animals and a socioeconomic problem. Enterobacterales, mainly Escherichia coli, carrying β-lactamases has become one of the main indicators to estimate the burden of AMR in animals within "One Health" approach.To assess the presence of extended-spectrum cephalosporin-resistant Enterobacterales associated with ruminants (cattle, sheep, goats) habituated in all five provinces of Rwanda and to perform in depth characterization of isolates.We screened 454 rectal swabs from 203 cows, 170 goats, and 81 sheep and selective isolation of extended-spectrum cephalosporin-resistant Enterobacterales was conducted. Isolates were identified as a members of the order Enterobacterales by MALDI-TOF MS and further characterized by susceptibility testing and by whole-genome sequencing.Out of the 454 samples, 64 extended-spectrum cephalosporin-resistant Enterobacterales were isolated from 58 animals. Isolates belonged to seven bacterial species and were identified as Escherichia coli (n = 54), Enterobacter bugandensis (n = 4), Enterobacter mori (n = 2), Klebsiella pneumoniae (n = 2), Enterobacter dykesii (n = 1), and Citrobacter freundii (n = 1). All isolates displayed an Extended-spectrum β-lactamases (ESBL) phenotype, with exception of Citrobacter freundii isolate displayed both an ESBL and AmpC phenotype. In addition, all Enterobacter isolates were identified as stably de-repressed AmpC-producers. ESBLs genes, blaCTX-M-15 was predominant. Resistance to tetracycline and tet(A) was most frequently observed among non-β-lactam resistance. Forty-eight isolates displayed multidrug-resistance phenotypes. A shiga toxin-producing E. coli and an enterotoxigenic E. coli isolate were observed. Genome comparisons revealed thirty-five E. coli sequence types (ST) (ST10, ST307 being predominate).Considering the high proximity between ruminants and humans in Rwanda, the dissemination of antimicrobial drug resistance highlights the public health threats and requires the joint and multisectoral action of human and veterinary medicine, at human-animal-environment interfaces. Therefore, it is important to establish national and global "One Health" surveillance programs of AMR to tackle the antibiotic-resistant crisis in human and veterinary medicine.

Keywords (eng)

AnimalsRwanda EpidemiologySheepAnti-Bacterial Agents PharmacologyEnterobacteriaceae Drug EffectsEnterobacteriaceae Isolation & PurificationGoatsCattleEnterobacteriaceae Infections VeterinaryEnterobacteriaceae Infections MicrobiologyEnterobacteriaceae Infections EpidemiologyCephalosporins PharmacologySheep Diseases MicrobiologySheep Diseases EpidemiologyGoat Diseases MicrobiologyGoat Diseases EpidemiologyPrevalenceMicrobial Sensitivity Tests VeterinaryCattle Diseases MicrobiologyCattle Diseases EpidemiologyDrug ResistanceBacterialbeta-Lactamases Genetics

Type (eng)

journal article

Language

[eng]

Persistent identifier

https://phaidra.vetmeduni.ac.at/o:3769

DOI

10.1186/s12917-024-04359-3

Is in series

Title (eng)

BMC Veterinary Research

Volume