Title
Characterization of the plasma proteome from healthy adult dogs
Language
English
Description (en)
Bloodwork is a widely used diagnostic tool in veterinary medicine, as diagnosis and therapeutic interventions often rely on blood biomarkers. However, biomarkers available in veterinary medicine often lack sensitivity or specificity. Mass spectrometry-based proteomics technology has been extensively used in the analysis of biological fluids. It offers excellent potential for a more comprehensive characterization of the plasma proteome in veterinary medicine.In this study, we aimed to identify and quantify plasma proteins in a cohort of healthy dogs and compare two techniques for depleting high-abundance plasma proteins to enable the detection of lower-abundance proteins via label-free quantification liquid chromatography-mass spectrometry. We utilized surplus lithium-heparin plasma from 30 healthy dogs, subdivided into five groups of pooled plasma from 6 randomly selected individuals each. Firstly, we used a commercial kit to deplete high-abundance plasma proteins. Secondly, we employed an in-house method to remove albumin using Blue-Sepharose.Among all the samples, some of the most abundant proteins identified were apolipoprotein A and B, albumin, alpha-2-macroglobulin, fibrinogen beta chain, fibronectin, complement C3, serotransferrin, and coagulation factor V. However, neither of the depletion techniques achieved significant depletion of highly abundant proteins. Despite this limitation, we could detect and quantify many clinically relevant proteins. Determining the healthy canine proteome is a crucial first step in establishing a reference proteome for canine plasma. After enrichment, this reference proteome can later be utilized to identify protein markers associated with different diseases, thereby contributing to the diagnosis and prognosis of various pathologies.
Keywords (en)
Mass-Spectrometry; Computational Platform; Canine Leishmaniasis; Biomarker Discovery; Proteins; Serum; Depletion; Fibrinogen; Fluid; Rat
DOI
10.3389/fvets.2024.1356318
Author of the digital object
Pavlos G.  Doulidis  (University of Veterinary Medicine Vienna)
Iwan A.  Burgener  (University of Veterinary Medicine Vienna)
Alexandro  Rodríguez-Rojas  (University of Veterinary Medicine Vienna)
Benno  Kuropka  (Freie Universität Berlin)
Carolina  Frizzo Ramos  (University of Veterinary Medicine Vienna)
Format
application/pdf
Size
4.0 MB
Licence Selected
CC BY 4.0 International
Type of publication
Article
Name of Publication (en)
Frontiers in Veterinary Science
Pages or Volume
9
Volume
11
Publisher
Frontiers Media Sa
Publication Date
2024
Content
Details
Object type
PDFDocument
Format
application/pdf
Created
17.10.2024 08:51:51
This object is in collection
Metadata
Veterinärmedizinische Universität Wien (Vetmeduni) | Veterinärplatz 1 | 1210 Wien - Österreich | T +43 1 25077-0 | Web: vetmeduni.ac.at