Titel (eng)

Deregulation of Metalloproteinase Expression in Gray Horse Melanoma Ex Vivo and In Vitro

Autor*in

Daniela M. Brodesser   University of Veterinary Medicine Vienna

Barbara Pratscher   University of Veterinary Medicine Vienna

Sabine Brandt   University of Veterinary Medicine Vienna

Christof A Bertram   University of Veterinary Medicine Vienna

Giuseppe Borzacchiello   University of Naples Federico II

Annunziata Corteggio   National Research Council of Italy

Karin Schlangen   Medical University of Vienna

Stefan Kummer   University of Veterinary Medicine Vienna

Julia A. Eichberger   University of Veterinary Medicine Vienna

Verlag

Cells

Beschreibung (eng)

The ability of human melanoma cells to switch from an epithelial to a mesenchymal phenotype contributes to the metastatic potential of disease. Metalloproteinases (MPs) are crucially involved in this process by promoting the detachment of tumor cells from the primary lesion and their migration to the vasculature. In gray horse melanoma, epithelial-mesenchymal transition (EMT) is poorly understood, prompting us to address MP expression in lesions versus intact skin by transcriptome analyses and the immunofluorescence staining (IF) of gray horse tumor tissue and primary melanoma cells. RNAseq revealed the deregulation of several MPs in gray horse melanoma and, notably, a 125-fold upregulation of matrix metalloproteinase 1 (MMP1) that was further confirmed by RT-qPCR from additional tumor material. The IF staining of melanoma tissue versus intact skin for MMP1 and tumor marker S100 revealed MMP1 expression in all lesions. The co-expression of S100 was observed at different extents, with some tumors scoring S100-negative. The IF staining of primary tumor cells explanted from the tumors for MMP1 showed that the metalloproteinase is uniformly expressed in the cytoplasm of 100% of tumor cells. Overall, the presented data point to MP expression being deregulated in gray horse melanoma, and suggest that MMP1 has an active role in gray horse melanoma by driving EMT-mediated tumor cell dissemination via the degradation of the extracellular matrix. Whilst S100 is considered a reliable tumor marker in human MM, gray horse melanomas do not seem to regularly express this protein.

Sprache des Objekts

Englisch

Datum

2024

Rechte

Dieses Werk bzw. dieser Inhalt steht unter einer
CC BY 4.0 - Creative Commons Namensnennung 4.0 International Lizenz.

CC BY 4.0 International

http://creativecommons.org/licenses/by/4.0/

Klassifikation

Animals; Melanomapathologyenzymologygeneticsmetabolism; Horses; Gene Expression Regulation, Neoplastic; Matrix Metalloproteinase 1metabolismgenetics; Epithelial-Mesenchymal Transitiongenetics; Skin Neoplasmspathologyenzymologygeneticsveterinarymetabolism; Cell Line, Tumor; Metalloproteasesmetabolismgenetics; Humans

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