Titel (eng)

Unexpected absence of exo-erythrocytic merogony during high gametocytaemia in two species of Haemoproteus (Haemosporida: Haemoproteidae), including description of Haemoproteus angustus n. sp. (lineage hCWT7) and a report of previously unknown residual bodies during in vitro gametogenesis

Autor*in

Gediminas Valkiūnas   Nature Research Centre

Herbert Weissenböck   University of Veterinary Medicine Vienna

Josef Harl   University of Veterinary Medicine Vienna

Tanja Himmel   University of Veterinary Medicine Vienna

Dovilė Bukauskaitė   Nature Research Centre

Tatjana Iezhova   Nature Research Centre

Mikas Ilgūnas   Nature Research Centre

Mélanie Tchoumbou   Nature Research Centre

Mélanie Duc   Nature Research Centre

Verlag

Elsevier

Beschreibung (eng)

Neglected avian blood parasites of the genus Haemoproteus (Haemoproteidae) have recently attracted attention due to the application of molecular diagnostic tools, which unravelled remarkable diversity of their exo-erythrocytic (or tissue) stages both regarding morphology and organ tropism levels. The development of haemoproteids might result in pathologies of internal organs, however the exo-erythrocytic development (EED) of most Haemoproteus species remains unknown. Seven individual birds - Curruca communis (1) and Phylloscopus trochilus (6) - with high gametocytaemia (between 1% and 24%) of Haemoproteus angustus n. sp. (hCWT7) and Haemoproteus palloris (lineage hWW1) were sampled in Lithuania, and their internal organs were examined extensively by parallel application of histology and chromogenic in situ hybridization methods. Tissue stages were apparently absent, suggesting that the parasitaemia was not accompanied by detectable tissue merogony. Haemoproteus angustus n. sp. was described and characterized morphologically and molecularly. Sexual process and ookinete development of the new species readily occurred in vitro, and a unique character for Haemoproteus parasites was discovered - the obligatory development of several tiny residual bodies, which were associated with intracellular transformation of both macrogametocytes and microgametocytes before their escape from the host cells and formation of gametes. A DNA haplotype network was constructed with lineages that cluster in one clade with the lineage hCWT7. This clade consists of lineages mostly found in Curruca birds, indicating specificity for birds of this genus. The lineage hCWT7 is mainly a parasite of C. communis. Most reports of this lineage came from Turkey, with only a few records in Europe, mostly in birds wintering in Africa where transmission probably occurs. This study highlights unexpected difficulties in the research of EED even when using sensitive molecular diagnostic tools and extends information about transformation in early stages of gametogenesis in haemosporidian parasites.

Sprache des Objekts

Englisch

Datum

2024

Rechte

Dieses Werk bzw. dieser Inhalt steht unter einer
CC BY-NC-ND 4.0 - Creative Commons Namensnennung - Nicht-kommerziell - Keine Bearbeitungen 4.0 International Lizenz.

CC BY-NC-ND 4.0 International

http://creativecommons.org/licenses/by-nc-nd/4.0/

Klassifikation

Aberrant Leucocytozoon Infection; Molecular Characterization; Hemoproteids Haemosporida; Plasmodium; Algorithm; Mortality; Parasites; Birds

Mitglied in der/den Collection(s) (1)