Titel (eng)

Comparative transcriptomics of the garden dormouse hypothalamus during hibernation

Autor*in

Elena Haugg   Ulm University

Annika Herwig   Ulm University

Sylvain Giroud   University of Veterinary Medicine Vienna / Northern Michigan University

Anna Kübber-Heiss   University of Veterinary Medicine Vienna

Janus Borner   American Museum of Natural History

Gabrielle Stalder   University of Veterinary Medicine Vienna

Verlag

Wiley

Beschreibung (eng)

Torpor or heterothermy is an energy-saving mechanism used by endotherms to overcome harsh environmental conditions. During winter, the garden dormouse (Eliomys quercinus) hibernates with multiday torpor bouts and body temperatures of a few degrees Celsius, interrupted by brief euthermic phases. This study investigates gene expression within the hypothalamus, the key brain area controlling energy balance, adding information on differential gene expression potentially relevant to orchestrate torpor. A de novo assembled transcriptome of the hypothalamus was generated from garden dormice hibernating under constant darkness without food and water at 5 °C. Samples were collected during early torpor, late torpor, and interbout arousal. During early torpor, 765 genes were differentially expressed as compared with interbout arousal. Twenty-seven pathways were over-represented, including pathways related to hemostasis, extracellular matrix organization, and signaling of small molecules. Only 82 genes were found to be differentially expressed between early and late torpor, and no pathways were over-represented. During late torpor, 924 genes were differentially expressed relative to interbout arousal. Despite the high number of differentially expressed genes, only 10 pathways were over-represented. Of these, eight were also observed to be over-represented when comparing early torpor and interbout arousal. Our results are largely consistent with previous findings in other heterotherms. The addition of a transcriptome of a novel species may help to identify species-specific and overarching torpor mechanisms through future species comparisons.

Sprache des Objekts

Englisch

Datum

2023

Rechte

Dieses Werk bzw. dieser Inhalt steht unter einer
CC BY 4.0 - Creative Commons Namensnennung 4.0 International Lizenz.

CC BY 4.0 International

http://creativecommons.org/licenses/by/4.0/

Klassifikation

Hepatocyte Growth-Factor; Daily Torpor; Gene-Expression; Suprachiasmatic Nucleus; Mammalian Hibernation; Extracellular-Matrix; Djungarian Hamsters; Seasonal Adaptation; Ambient-Temperature; Body-Temperature

Mitglied in der/den Collection(s) (1)