Titel (eng)

RNAi-directed knockdown in the cnidarian fish blood parasite Sphaerospora molnari

Autor*in

Jiří Kyslík   Academy of Sciences of the Czech Republic

Anush Kosakyan   Academy of Sciences of the Czech Republic / University of Modena and Reggio Emilia / National Biodiversity Future Center

Ana Born-Torrijos   Academy of Sciences of the Czech Republic / NIOZ Royal Netherlands Institute for Sea Research

Astrid S. Holzer   University of Veterinary Medicine Vienna / Academy of Sciences of the Czech Republic

Verlag

Nature Portfolio

Beschreibung (eng)

RNA interference (RNAi) is an effective approach to suppress gene expression and monitor gene regulation. Despite its wide application, its use is limited in certain taxonomic groups, including cnidarians. Myxozoans are a unique group of cnidarian parasites that diverged from their free-living ancestors about 600 million years ago, with several species causing acute disease in farmed and wild fish populations. In this pioneering study we successfully applied RNAi in blood stages of the myxozoan Sphaerospora molnari, combining a dsRNA soaking approach, real-time PCR, confocal microscopy, and Western blotting. For proof of concept, we knocked down two unusual actins, one of which is known to play a critical role in S. molnari cell motility. We observed intracellular uptake of dsRNA after 30 min and accumulation in all cells of the typical myxozoan cell-in-cell structure. We successfully knocked down actin in S. molnari in vitro, with transient inhibition for 48 h. We observed the disruption of the cytoskeletal network within the primary cell and loss of the characteristic rotational cell motility. This RNAi workflow could significantly advance functional research within the Myxozoa, offering new prospects for investigating therapeutic targets and facilitating drug discovery against economically important fish parasites.

Sprache des Objekts

Englisch

Datum

2024

Rechte

Dieses Werk bzw. dieser Inhalt steht unter einer
CC BY 4.0 - Creative Commons Namensnennung 4.0 International Lizenz.

CC BY 4.0 International

http://creativecommons.org/licenses/by/4.0/

Klassifikation

Animals; Cnidariagenetics; Parasites; RNA Interference; Myxozoagenetics; Cell Movement; Fishes; Actinsgenetics; Fish Diseasesgenetics; Phylogeny

Mitglied in der/den Collection(s) (1)