Titel (eng)

Antimicrobial Peptides (AMP) in the Cell-Free Culture Media of Xenorhabdus budapestensis and X. szentirmaii Exert Anti-Protist Activity against Eukaryotic Vertebrate Pathogens including Histomonas meleagridis and Leishmania donovani Species

Autor*in

András Fodor   Eötvös Loránd University

Michael Hess   University of Veterinary Medicine Vienna

Tibor Vellai   Eötvös Loránd University

Bradford S. McGwire   The Ohio State University

Manjusha M. Kulkarni   The Ohio State University

Eustachio Tarasco   University of Bari "Aldo Moro"

Michael G. Klein   The Ohio State University

Anna Sebestyén   Semmelweis University

László Fodor   University of Veterinary Medicine Vienna

László Pál   Hungarian University of Agriculture and Life Sciences

Károly Dublecz   Hungarian University of Agriculture and Life Sciences

László Makrai   Autovakcina Kft

János Kiss   Hungarian University of Agriculture and Life Sciences

Zsófia Boros   Eötvös Loránd University , Hungarian University of Agriculture and Life Sciences

Claudia Hess   University of Veterinary Medicine Vienna

Petra Ganas   University of Veterinary Medicine Vienna

Verlag

MDPI

Beschreibung (eng)

Anti-microbial peptides provide a powerful toolkit for combating multidrug resistance. Combating eukaryotic pathogens is complicated because the intracellular drug targets in the eukaryotic pathogen are frequently homologs of cellular structures of vital importance in the host organism. The entomopathogenic bacteria (EPB), symbionts of entomopathogenic-nematode species, release a series of non-ribosomal templated anti-microbial peptides. Some may be potential drug candidates. The ability of an entomopathogenic-nematode/entomopathogenic bacterium symbiotic complex to survive in a given polyxenic milieu is a coevolutionary product. This explains that those gene complexes that are responsible for the biosynthesis of different non-ribosomal templated anti-microbial protective peptides (including those that are potently capable of inactivating the protist mammalian pathogen Leishmania donovanii and the gallinaceous bird pathogen Histomonas meleagridis) are co-regulated. Our approach is based on comparative anti-microbial bioassays of the culture media of the wild-type and regulatory mutant strains. We concluded that Xenorhabdus budapestensis and X. szentirmaii are excellent sources of non-ribosomal templated anti-microbial peptides that are efficient antagonists of the mentioned pathogens. Data on selective cytotoxicity of different cell-free culture media encourage us to forecast that the recently discovered "easy-PACId" research strategy is suitable for constructing entomopathogenic-bacterium (EPB) strains producing and releasing single, harmless, non-ribosomal templated anti-microbial peptides with considerable drug, (probiotic)-candidate potential.

Sprache des Objekts

Englisch

Datum

2023

Rechte

Dieses Werk bzw. dieser Inhalt steht unter einer
CC BY 4.0 - Creative Commons Namensnennung 4.0 International Lizenz.

CC BY 4.0 International

http://creativecommons.org/licenses/by/4.0/

Klassifikation

Escherichia-Coli; In-Vitro; Tetratrichomonas-Gallinarum; Bacteria Xenorhabdus; Resistance; Poultry; Disease; Hfq; Establishment; Depsipeptide

Mitglied in der/den Collection(s) (1)