Antimicrobial Peptides (AMP) in the Cell-Free Culture Media of Xenorhabdus budapestensis and X. szentirmaii Exert Anti-Protist Activity against Eukaryotic Vertebrate Pathogens including Histomonas meleagridis and Leishmania donovani Species
Eötvös Loránd University
University of Veterinary Medicine Vienna
Tibor Vellai Eötvös Loránd University
Bradford S. McGwire The Ohio State University
Manjusha M. Kulkarni The Ohio State University
University of Bari "Aldo Moro"
Michael G. Klein The Ohio State University
Anna Sebestyén Semmelweis University
University of Veterinary Medicine Vienna
Hungarian University of Agriculture and Life Sciences
Hungarian University of Agriculture and Life Sciences
László Makrai Autovakcina Kft
Hungarian University of Agriculture and Life Sciences
Zsófia Boros Eötvös Loránd University , Hungarian University of Agriculture and Life Sciences
University of Veterinary Medicine Vienna
Petra Ganas University of Veterinary Medicine Vienna
MDPI
Anti-microbial peptides provide a powerful toolkit for combating multidrug resistance. Combating eukaryotic pathogens is complicated because the intracellular drug targets in the eukaryotic pathogen are frequently homologs of cellular structures of vital importance in the host organism. The entomopathogenic bacteria (EPB), symbionts of entomopathogenic-nematode species, release a series of non-ribosomal templated anti-microbial peptides. Some may be potential drug candidates. The ability of an entomopathogenic-nematode/entomopathogenic bacterium symbiotic complex to survive in a given polyxenic milieu is a coevolutionary product. This explains that those gene complexes that are responsible for the biosynthesis of different non-ribosomal templated anti-microbial protective peptides (including those that are potently capable of inactivating the protist mammalian pathogen Leishmania donovanii and the gallinaceous bird pathogen Histomonas meleagridis) are co-regulated. Our approach is based on comparative anti-microbial bioassays of the culture media of the wild-type and regulatory mutant strains. We concluded that Xenorhabdus budapestensis and X. szentirmaii are excellent sources of non-ribosomal templated anti-microbial peptides that are efficient antagonists of the mentioned pathogens. Data on selective cytotoxicity of different cell-free culture media encourage us to forecast that the recently discovered "easy-PACId" research strategy is suitable for constructing entomopathogenic-bacterium (EPB) strains producing and releasing single, harmless, non-ribosomal templated anti-microbial peptides with considerable drug, (probiotic)-candidate potential.
Englisch
2023
Dieses Werk bzw. dieser Inhalt steht unter einer
CC BY 4.0 - Creative Commons Namensnennung 4.0 International Lizenz.
CC BY 4.0 International
http://creativecommons.org/licenses/by/4.0/
Escherichia-Coli; In-Vitro; Tetratrichomonas-Gallinarum; Bacteria Xenorhabdus; Resistance; Poultry; Disease; Hfq; Establishment; Depsipeptide