Cross-species communication via agr controls phage susceptibility in Staphylococcus aureus
Jingxian Yang University of Copenhagen
Hanne Ingmer University of Copenhagen
Andreas Peschel University of Tübingen / German Center for Infection Research
University of Veterinary Medicine Vienna
Martin Saxtorph Bojer University of Copenhagen
Stephanie Fulaz Silva University of Copenhagen
Janine Zara Bowring University of Copenhagen
Janes Krusche University of Tübingen / German Center for Infection Research
Esther Lehmann University of Copenhagen
Benjamin Svejdal Bejder University of Copenhagen
Cell Press
Bacteria use quorum sensing (QS) to coordinate group behavior in response to cell density, and some bacterial viruses (phages) also respond to QS. In Staphylococcus aureus, the agr-encoded QS system relies on accumulation of auto-inducing cyclic peptides (AIPs). Other staphylococci also produce AIPs of which many inhibit S. aureus agr. We show that agr induction reduces expression of tarM, encoding a glycosyltransferase responsible for α-N-acetylglucosamine modification of the major S. aureus phage receptor, the wall teichoic acids. This allows lytic phage Stab20 and related phages to infect and kill S. aureus. However, in mixed communities, producers of inhibitory AIPs like S. haemolyticus, S. caprae, and S. pseudintermedius inhibit S. aureus agr, thereby impeding phage infection. Our results demonstrate that cross-species interactions dramatically impact phage susceptibility. These interactions likely influence microbial ecology and impact the efficacy of phages in medical and biotechnological applications such as phage therapy.
Englisch
2023
Dieses Werk bzw. dieser Inhalt steht unter einer
CC BY-NC-ND 4.0 - Creative Commons Namensnennung - Nicht-kommerziell - Keine Bearbeitungen 4.0 International Lizenz.
CC BY-NC-ND 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
Humans; Staphylococcus aureusmetabolism; Bacteriophagesmetabolism; Staphylococcusmetabolism; Glycosyltransferasesmetabolism; Staphylococcal Infections; Bacterial Proteinsmetabolism; Quorum Sensing