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Title (en)
Novel Ehrlichia canis genogroup in dogs with canine ehrlichiosis in Cuba
Language
English
Description (en)
Canine monocytic ehrlichiosis (CME) is caused by the tick-borne pathogen Ehrlichia canis, an obligate intracellular Gram-negative bacterium of the family Anaplasmataceae with tropism for canine monocytes and macrophages. The trp36 gene, which encodes for the major immunoreactive protein TRP36 in E. canis, has been successfully used to characterize the genetic diversity of this pathogen in different regions of the world. Based on trp36 sequence analysis, four E. canis genogroups, United States (US), Taiwan (TWN), Brazil (BR) and Costa Rica (CR), have been identified. The aim of this study was to characterize the genetic diversity of E. canis in Cuba based on the trp36 gene.Whole blood samples (n = 8) were collected from dogs found to be infested with the tick vector Rhipicephalus sanguineus sensu lato (s.l.) and/or presenting clinical signs and symptoms of CME. Total DNA was extracted from the blood samples and trp36 fragments were amplified by PCR. Nucleotide and protein sequences were compared using alignments and phylogenetic analysis.Four of the trp36 sequences obtained (n = 8) fall within the phylogenetic cluster grouping the US genogroup E. canis strains. The other E. canis trp36 sequences formed a separate and well-supported clade (94% bootstrap value) that is phylogenetically distant from the other major groups and thus represents a new genogroup, herein designated as the 'Cuba (CUB) genogroup'. Notably, dogs infected with the CUB genogroup presented frequent hemorrhagic lesions.The results of this study suggest that genetic diversification of E. canis in Cuba is associated with the emergence of E. canis strains with increased virulence.
Keywords (en)
Animals; Cuba; Dog Diseasesmicrobiology; Dogs; Ehrlichia; Ehrlichia canisgenetics; Ehrlichiosismicrobiologyveterinary; Genotype; Phylogeny
DOI
10.1186/s13071-022-05426-0
Author of the digital object
Maylin González Navarrete  (Agrarian University of Havana)
Alejandro Cabezas-Cruz  (Université Paris-Est)
Adivaldo Henrique da Fonseca  (Federal Rural University of Rio de Janeiro)
Lianet Abuin-Denis  (Center for Genetic Engineering and Biotechnology Cuba)
Elianne Piloto-Sardiñas  (National Center for Animal and Plant Health Cuba)
Eugenio Roque López  (Agrarian University of Havana )
Alejandra Wu-Chuang  (Université Paris-Est)
Ísis Indaiara Gonçalves Granjeiro Taques  (Federal University of Mato Grosso State)
Amanda Noeli da Silva Campos  (Federal University of Mato Grosso State)
Daniel Moura de Aguiar  (Federal University of Mato Grosso State)
Dasiel Obregón  (University of Guelph)
Liani Coronado Báez  (National Center for Animal and Plant Health Cuba)
Claudia Bezerra da Silva  (Federal Rural University of Rio de Janeiro)
Matheus Dias Cordeiro  (Federal Rural University of Rio de Janeiro)
Belkis Corona-González  (National Center for Animal and Plant Health Cuba)
Adnan Hodžić  (University of Veterinary Medicine Vienna)
Format
application/pdf
Size
1.5 MB
Licence Selected
CC BY 4.0 International
Type of publication
Article
Name of Publication (en)
Parasites & Vectors
Pages or Volume
9
Volume
15
Number
1
Publisher
BMC
Publication Date
2022