Titel (eng)

Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov.

Autor*in

Ivo Sedláček   Masaryk University

Karel Sedlář   Brno University of Technology / Ludwig-Maximilians-Universität München

Jana Smolíková   Charles University

Ondrej Šedo   Masaryk University

Jan Pilný   Czech Academy of Sciences

Eva Staňková   Masaryk University

Roman Sobotka   Czech Academy of Sciences

Pavel Švec   Masaryk University

Stanislava Králová   Masaryk University

Vendula Koublová   Masaryk University

Pavla Holochová   Masaryk University

Hans-Jürgen Busse   University of Veterinary Medicine Vienna

Verlag

MDPI

Beschreibung (eng)

A group of seven bacterial strains producing blue-purple pigmented colonies on R2A agar was isolated from freshwater samples collected in a deglaciated part of James Ross Island and Eagle Island, Antarctica, from 2017-2019. The isolates were psychrophilic, oligotrophic, resistant to chloramphenicol, and exhibited strong hydrolytic activities. To clarify the taxonomic position of these isolates, a polyphasic taxonomic approach was applied based on sequencing of the 16S rRNA, gyrB and lepA genes, whole-genome sequencing, rep-PCR, MALDI-TOF MS, chemotaxonomy analyses and biotyping. Phylogenetic analysis of the 16S rRNA gene sequences revealed that the entire group are representatives of the genus Massilia. The closest relatives of the reference strain P8398T were Massilia atriviolacea, Massilia violaceinigra, Massilia rubra, Massilia mucilaginosa, Massilia aquatica, Massilia frigida, Massilia glaciei and Massilia eurypsychrophila with a pairwise similarity of 98.6-100% in the 16S rRNA. The subsequent gyrB and lepA sequencing results showed the novelty of the analysed group, and the average nucleotide identity and digital DNA-DNA hybridisation values clearly proved that P8398T represents a distinct Massilia species. After all these results, we nominate a new species with the proposed name Massilia antarctica sp. nov. The type strain is P8398T (= CCM 8941T = LMG 32108T).

Sprache des Objekts

Englisch

Datum

2022

Rechte

Dieses Werk bzw. dieser Inhalt steht unter einer
CC BY 4.0 - Creative Commons Namensnennung 4.0 International Lizenz.

CC BY 4.0 International

http://creativecommons.org/licenses/by/4.0/

Klassifikation

Emended Description; Lipid-Composition; Bacteria; Genome; Database; Soil; Gene; Classification; Degradation; Restriction

Mitglied in der/den Collection(s) (1)