Titel (eng)

The Natural Cytotoxicity Receptor NKp44 (NCR2, CD336) Is Expressed on the Majority of Porcine NK Cells Ex Vivo Without Stimulation

Autor*in

Kerstin H. Mair   University of Veterinary Medicine Vienna

Joan K. Lunney   United States Department of Agriculture

Armin Saalmüller   University of Veterinary Medicine Vienna

Wilhelm Gerner   University of Veterinary Medicine Vienna / The Pirbright Institute

Katinka A van Dongen   University of Veterinary Medicine Vienna

Maria Stadler   University of Veterinary Medicine Vienna

Dante Zarlenga   United States Department of Agriculture

Patricia Boyd   United States Department of Agriculture

Leela Noronha   Cornell University / United States Department of Agriculture

Susanna Babasyan   Cornell University

Bettina Wagner   Cornell University

Assiatu J. Crossman   National Institutes of Health / United States Department of Agriculture

Verlag

Frontiers Media Sa

Beschreibung (eng)

Natural killer (NK) cells have been studied extensively in humans and mice for their vital role in the vertebrate innate immune system. They are known to rapidly eliminate tumors or virus infected cells in an immune response utilizing their lytic properties. The natural cytotoxicity receptors (NCRs) NKp30 (NCR3), NKp44 (NCR2), and NKp46 (NCR1) are important mediators of NK-cell cytotoxicity. NKp44 expression was reported for NK cells in humans as well as in some non-human primates and found exclusively on activated NK cells. Previously, no information was available on NKp44 protein expression and its role in porcine lymphocytes due to the lack of species-specific monoclonal antibodies (mAbs). For this study, porcine-specific anti-NKp44 mAbs were generated and their reactivity was tested on blood and tissue derived NK cells in pigs of different age classes. Interestingly, NKp44 expression was detected ex vivo already on resting NK cells; moreover, the frequency of NKp44+ NK cells was higher than that of NKp46+ NK cells in most animals analyzed. Upon in vitro stimulation with IL-2 or IL-15, the frequency of NKp44+ NK cells, as well as the intensity of NKp44 expression at the single cell level, were increased. Since little is known about swine NK cells, the generation of a mAb (clone 54-1) against NKp44 will greatly aid in elucidating the mechanisms underlying the differentiation, functionality, and activation of porcine NK cells.

Sprache des Objekts

Englisch

Datum

2022

Rechte

Dieses Werk bzw. dieser Inhalt steht unter einer
CC BY 4.0 - Creative Commons Namensnennung 4.0 International Lizenz.

CC BY 4.0 International

http://creativecommons.org/licenses/by/4.0/

Klassifikation

Killer-Cells; Monoclonal-Antibodies; Activation; Recognition; Infection; Subsets; Nkp30; Pigs; Ige

Mitglied in der/den Collection(s) (1)