Titel (eng)

Impact of a Novel PagR-like Transcriptional Regulator on Cereulide Toxin Synthesis in Emetic Bacillus cereus

Autor*in

Eva Maria Kalbhenn   University of Veterinary Medicine Vienna

Monika Ehling-Schulz   University of Veterinary Medicine Vienna

Elrike Frenzel   University of Veterinary Medicine Vienna

Timo D. Stark   Technical University of Munich

Gregor Grass   Bundeswehr Institute of Microbiology

Markus Kranzler   University of Veterinary Medicine Vienna

Agnieszka Gacek-Matthews   University of Veterinary Medicine Vienna

Verlag

MDPI

Beschreibung (eng)

The emetic type of foodborne disease caused by Bacillus cereus is produced by the small peptide toxin cereulide. The genetic locus encoding the Ces nonribosomal peptide synthetase (CesNRPS) multienzyme machinery is located on a 270 kb megaplasmid, designated pCER270, which shares its backbone with the Bacillus anthracis toxin plasmid pXO1. Although the ces genes are plasmid-borne, the chromosomally encoded pleiotropic transcriptional factors CodY and AbrB are key players in the control of ces transcription. Since these proteins only repress cereulide synthesis during earlier growth phases, other factors must be involved in the strict control of ces expression and its embedment in the bacterial life cycle. In silico genome analysis revealed that pCER270 carries a putative ArsR/SmtB family transcription factor showing high homology to PagR from B. anthracis. As PagR plays a crucial role in the regulation of the protective antigen gene pagA, which forms part of anthrax toxin, we used a gene-inactivation approach, combined with electrophoretic mobility shift assays and a bacterial two-hybrid system for dissecting the role of the PagR homologue PagRBc in the regulation of cereulide synthesis. Our results highlight that the plasmid-encoded transcriptional regulator PagRBc plays an important role in the complex and multilayered process of cereulide synthesis.

Sprache des Objekts

Englisch

Datum

2022

Rechte

Dieses Werk bzw. dieser Inhalt steht unter einer
CC BY 4.0 - Creative Commons Namensnennung 4.0 International Lizenz.

CC BY 4.0 International

http://creativecommons.org/licenses/by/4.0/

Klassifikation

Escherichia-Coli; Identification; Virulence; Protein; Expression; Anthracis; Family; Repressors; Plasmids; System

Mitglied in der/den Collection(s) (1)