Titel (eng)

XENOFOOD-An Autoclaved Feed Supplement Containing Autoclavable Antimicrobial Peptides-Exerts Anticoccidial GI Activity, and Causes Bursa Enlargement, but Has No Detectable Harmful Effects in Broiler Cockerels despite In Vitro Detectable Cytotoxicity on LHM Cells

Autor*in

András Fodor   Eötvös Loránd University

Michael Hess   University of Veterinary Medicine Vienna

Petra Ganas   University of Veterinary Medicine Vienna

Sándor Józsa   Hungarian University of Agriculture and Life Sciences

Eustachio Tarasco   University of Bari "Aldo Moro"

Michael G. Klein   The Ohio State University

Andor Molnár   Hungarian University of Agriculture and Life Sciences

László Pál   Hungarian University of Agriculture and Life Sciences

Károly Dublecz   Hungarian University of Agriculture and Life Sciences

László Makrai   University of Veterinary Medicine, Budapest

Claudia Hess   University of Veterinary Medicine Vienna

Tibor Vellai   Eötvös Loránd University

Verlag

MDPI

Beschreibung (eng)

Entomopathogenic bacteria are obligate symbionts of entomopathogenic nematode (EPN) species. These bacteria biosynthesize and release non-ribosomal-templated hybrid peptides (NR-AMPs), with strong, and large-spectral antimicrobial potential, capable of inactivating pathogens belonging to different prokaryote, and eukaryote taxa. The cell-free conditioned culture media (CFCM) of Xenorhabdus budapestensis and X. szentirmaii efficiently inactivate poultry pathogens like Clostridium, Histomonas, and Eimeria. To learn whether a bio-preparation containing antimicrobial peptides of Xenorhabdus origin with accompanying (in vitro detectable) cytotoxic effects could be considered a safely applicable preventive feed supplement, we conducted a 42-day feeding experiment on freshly hatched broiler cockerels. XENOFOOD (containing autoclaved X. budapestensis, and X. szentirmaii cultures developed on chicken food) were consumed by the birds. The XENOFOOD exerted detectable gastrointestinal (GI) activity (reducing the numbers of the colony-forming Clostridium perfringens units in the lower jejunum. No animal was lost in the experiment. Neither the body weight, growth rate, feed-conversion ratio, nor organ-weight data differed between the control (C) and treated (T) groups, indicating that the XENOFOOD diet did not result in any detectable adverse effects. We suppose that the parameters indicating a moderate enlargement of bursas of Fabricius (average weight, size, and individual bursa/spleen weight-ratios) in the XENOFOOD-fed group must be an indirect indication that the bursa-controlled humoral immune system neutralized the cytotoxic ingredients of the XENOFOOD in the blood, not allowing to reach their critical cytotoxic concentration in the sensitive tissues.

Sprache des Objekts

Englisch

Datum

2023

Rechte

Dieses Werk bzw. dieser Inhalt steht unter einer
CC BY 4.0 - Creative Commons Namensnennung 4.0 International Lizenz.

CC BY 4.0 International

http://creativecommons.org/licenses/by/4.0/

Klassifikation

Xenorhabdus-Szentirmaii; Natural-Products; Enterobacteriaceae; Rhabdopeptides; Depsipeptide; Antibiotics; Szentiamide; Metabolite; Virulence; Family

Mitglied in der/den Collection(s) (1)