Titel (eng)

Zearalenone and Its Emerging Metabolites Promptly Affect the Rumen Microbiota in Holstein Cows Fed a Forage-Rich Diet

Autor*in

Thomas Hartinger   University of Veterinary Medicine, Vienna

Qendrim Zebeli   University of Veterinary Medicine, Vienna

Dian Schatzmayr   Biomin Holding GmbH

Barbara Doupovec   Biomin Holding GmbH

Johannes Faas   Biomin Holding GmbH

Iris Kröger   University of Veterinary Medicine, Vienna

Viktoria Neubauer   University of Veterinary Medicine, Vienna

Verlag

MDPI

Beschreibung (eng)

The study investigated the short-term effects of a single oral bolus of zearalenone (ZEN) on the rumen microbiota and fermentation patterns in four rumen-cannulated Holstein cows fed a forage diet with daily 2 kg/cow concentrate. During the baseline day, cows received uncontaminated concentrate, followed by ZEN-contaminated concentrate on the second day, and again the uncontaminated concentrate on day three. Free rumen liquid (FRL) and particle-associated rumen liquid (PARL) were collected at different hours post-feeding on all days to analyze the prokaryotic community composition, absolute abundances of bacteria, archaea, protozoa, and anaerobic fungi, as well as short-chain fatty acid (SCFA) profiles. The ZEN reduced the microbial diversity in FRL but not in the PARL fraction. The abundance of protozoa was higher after ZEN exposure in PARL, which may be related to their strong biodegradation capacity that, therefore, promoted protozoal growth. In contrast, α-zearalenol might compromise anaerobic fungi as indicated by reduced abundances in FRL and fairly negative correlations in both fractions. Total SCFA significantly increased in both fractions after ZEN exposure, while the SCFA profile only changed marginally. Concluding, a single ZEN challenge caused changes in the rumen ecosystem soon after intake, including ruminal eukaryotes, that should be the subject of future studies.

Sprache des Objekts

Englisch

Datum

2023

Rechte

Dieses Werk bzw. dieser Inhalt steht unter einer
CC BY 4.0 - Creative Commons Namensnennung 4.0 International Lizenz.

CC BY 4.0 International

http://creativecommons.org/licenses/by/4.0/

Klassifikation

Female; Cattle; Animals; Zearalenonetoxicitymetabolism; Rumenmetabolism; Dietveterinary; Fatty Acids, Volatilemetabolism; Microbiota; Fermentation; Animal Feedanalysis; Lactationmetabolism

Mitglied in der/den Collection(s) (1)