Title (eng)

Evidence-Based Severity Assessment of Animal Models for Pancreatic Cancer

Author

Tim Schreiber   Rostock University Medical Center

Simone Kumstel   Rostock University Medical Center

Brigitte Vollmar   Rostock University Medical Center

Dietmar Zechner   Rostock University Medical Center

Rupert Palme   University of Veterinary Medicine Vienna

Robert Jaster   Rostock University Medical Center

Bernd Krause   Rostock University Medical Center

Markus Joksch   Rostock University Medical Center

Anna Schildt   Rostock University Medical Center

Lisa Hoffmann   Rostock University Medical Center

Lea Goldstein   Rostock University Medical Center

Steven R. Talbot   Medizinische Hochschule Hannover

Ingo Koopmann   Rostock University Medical Center

Jakob Brandstetter   Rostock University Medical Center

Publishing

MDPI

Description (eng)

Animal models are crucial to preclinical oncological research and drug development. Animal experiments must be performed in accordance with the 3R principles of replacement and reduction, if possible, and refinement where these procedures remain crucial. In addition, European Union legislations demand a continuous refinement approach, as well as pro- and retrospective severity assessment. In this study, an objective databased severity assessment was performed in murine models for pancreatic cancer induced by orthotopic, subcutaneous, or intravenous injection of Panc02 cells. Parameters such as body weight change, distress score, perianal temperature, mouse grimace scale, burrowing, nesting behavior, and the concentration of corticosterone in plasma and its metabolites in feces were monitored during tumor progression. The most important parameters were combined into a score and mapped against a reference data set by the Relative Severity Assessment procedure (RELSA) to obtain the maximum achieved severity for each animal (RELSAmax). This scoring revealed a significantly higher RELSAmax for the orthotopic model than for the subcutaneous and intravenous models. However, compared to animal models such as pancreatitis and bile duct ligation, the pancreatic cancer models are shown to be less severe. Data-based animal welfare assessment proved to be a valuable tool for comparing the severity of differently induced cancer models.

Object languages

English

Date

2024

Rights

This work is licensed under a
CC BY 4.0 - Creative Commons Attribution 4.0 International License.

CC BY 4.0 International

http://creativecommons.org/licenses/by/4.0/

Classification

Working Group; Tumor-Models; Murine Model; End-Points; Mouse; Discovery; Pain; Mice; Opportunities; Metastasis

Member of the Collection(s) (1)