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Title (en)
Diversity of Staphylococcus aureus associated with mastitis from dairy cows in Rwanda
Language
English
Description (en)
The objective of the present study was to examine the diversity of Staphylococcus aureus from mastitis milk samples of cows in Rwanda.A total of 1080 quarter milk samples from 279 dairy cows were collected in 80 different farms from all five provinces of Rwanda. In total, 135 S. aureus isolates were obtained and subjected to genotyping (spa typing, DNA microarray, whole-genome sequencing (WGS)), antimicrobial susceptibility testing (AST) and phenotypic profiling by Fourier Transform Infrared (FTIR) spectroscopy (including capsular serotyping).Resistance to penicillin and/or tetracycline was most frequently observed. Ten sequence types (STs) (ST1, ST151, ST152, ST5477, ST700, ST7110, ST7983, ST7984, ST8320, ST97) belonging to seven clonal complexes (CCs) (CC1, CC130, CC152, CC3591, CC3666, CC705, CC97) were detected. The Panton-Valentine leukocidin (PVL) genes (lukF-PV/lukS-PV), the bovine leukocidin genes (lukM/lukF-P83) and the human and bovine toxic shock syndrome toxin gene tst-1 variants were detected. FTIR-based capsular serotyping showed CC-specific differences. Most CC97 (cap5 allele) isolates were primarily nonencapsulated (82%), whereas isolates of CC3591 and CC3666 (cap8 allele) were mostly encapsulated (86.4% and 57.8%, respectively). Our results underline the widespread global distribution of cattle-adapted CC97.The presence of CC3591 and CC3666 in bovine mastitis suggests an important role in cattle health and dairy production in Rwanda. The results of the present study support the need for a rigorous One-Health Surveillance program of the bovine-human interface.
Keywords (en)
Female; Cattle; Animals; Humans; Staphylococcus aureus; Rwandaepidemiology; Staphylococcal Infectionsepidemiologyveterinary; Anti-Bacterial Agentspharmacology; Mastitis
DOI
10.1016/j.jgar.2024.01.017
Author of the digital object
Helga Keinprecht  (University of Veterinary Medicine Vienna / New Vision Veterinary Hospital)
Igor Loncaric  (University of Veterinary Medicine Vienna)
Tom Grunert  (University of Veterinary Medicine Vienna)
Werner Ruppitsch  (Austrian Agency for Health and Food Safety)
Ralf Ehricht  (Leibniz Institute of Photonic Technology / Friedrich Schiller University Jena)
Stefan Monecke  (Leibniz Institute of Photonic Technology / Universitätsklinik Dresden)
Monika Ehling-Schulz  (University of Veterinary Medicine Vienna)
Joachim Spergser  (University of Veterinary Medicine Vienna)
Stefan Schwarz  (Freie Universität Berlin)
Sascha D. Braun  (Leibniz Institute of Photonic Technology / InfectoGnostics Research Campus)
Andrea T. Feßler  (Freie Universität Berlin)
Amelie Desvars-Larrive  (University of Veterinary Medicine Vienna / Complexity Science Hub Vienna)
Elke Müller  (Leibniz Institute of Photonic Technology / InfectoGnostics Research Campus)
Thomas Wittek  (University of Veterinary Medicine Vienna)
Jennifer Zöchbauer  (University of Veterinary Medicine Vienna)
Michael P. Szostak  (University of Veterinary Medicine Vienna)
Otto W. Fischer  (New Vision Veterinary Hospital)
Lydia Marek  (University of Veterinary Medicine Vienna / New Vision Veterinary Hospital)
Emmanuel Irimaso  (University of Rwanda)
Adriana Cabal Rosel  (Austrian Agency for Health and Food Safety)
Beatrix Stessl  (University of Veterinary Medicine Vienna)
Christophe Ntakirutimana  (New Vision Veterinary Hospital)
Format
application/pdf
Size
1.5 MB
Licence Selected
CC BY-NC-ND 4.0 International
Type of publication
Article
Name of Publication (en)
Journal of Global Antimicrobial Resistance
Pages or Volume
10
Volume
36
From Page
326
To Page
335
Publisher
Elsevier
Publication Date
2024