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Title (en)
Neutralising Effects of Different Antibodies on Clostridioides difficile Toxins TcdA and TcdB in a Translational Approach
Language
English
Description (en)
Given the high prevalence of intestinal disease in humans and animals, there is a strong need for clinically relevant models recapitulating gastrointestinal systems, ideally replacing in vivo models in accordance with the principles of the 3R. We established a canine organoid system and analysed the neutralising effects of recombinant versus natural antibodies on Clostridioides difficile toxins A and B in this in vitro system. Sulforhodamine B cytotoxicity assays in 2D and FITC-dextran barrier integrity assays on basal-out and apical-out organoids revealed that recombinant, but not natural antibodies, effectively neutralised C. difficile toxins. Our findings emphasise that canine intestinal organoids can be used to test different components and suggest that they can be further refined to also mirror complex interactions between the intestinal epithelium and other cells.
DOI
10.3390/ijms24043867
Author of the digital object
Georg Csukovich  (University of Veterinary Medicine, Vienna)
Nina Kramer  (University of Veterinary Medicine, Vienna)
Barbara Pratscher  (University of Veterinary Medicine, Vienna)
Ivana Gotic  (The Antibody Lab GmbH)
Patricia Freund  (University of Veterinary Medicine, Vienna)
Rainer Hahn  (University of Natural Resources and Life Sciences Vienna)
Gottfried Himmler  (The Antibody Lab GmbH)
Sabine Brandt  (University of Veterinary Medicine, Vienna)
Iwan Anton Burgener  (University of Veterinary Medicine, Vienna)
Format
application/pdf
Size
2.8 MB
Licence Selected
CC BY 4.0 International
Type of publication
Article
Name of Publication (en)
International Journal of Molecular Sciences
Pages or Volume
15
Volume
24
Number
4
Publisher
MDPI
Publication Date
2023