
<resource xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:datacite="http://datacite.org/schema/kernel-4" xmlns="http://namespace.openaire.eu/schema/oaire/" xsi:schemaLocation="http://namespace.openaire.eu/schema/oaire/ https://www.openaire.eu/schema/repo-lit/4.0/openaire.xsd">
  
<datacite:identifier identifierType="URL">https://phaidra.vetmeduni.ac.at/o:1377</datacite:identifier>

  
<datacite:titles>
  
<datacite:title xml:lang="en">Neutralising Effects of Different Antibodies on Clostridioides difficile Toxins TcdA and TcdB in a Translational Approach</datacite:title>

  
</datacite:titles>

  
<datacite:creators>
  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Csukovich, Georg</datacite:creatorName>

  
<datacite:givenName>Georg</datacite:givenName>

  
<datacite:familyName>Csukovich</datacite:familyName>

  
<datacite:nameIdentifier nameIdentifierScheme="ORCID" schemeURI="https://orcid.org/">0000-0001-5615-666X</datacite:nameIdentifier>

  
<datacite:affiliation>University of Veterinary Medicine Vienna</datacite:affiliation>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Kramer, Nina</datacite:creatorName>

  
<datacite:givenName>Nina</datacite:givenName>

  
<datacite:familyName>Kramer</datacite:familyName>

  
<datacite:nameIdentifier nameIdentifierScheme="ORCID" schemeURI="https://orcid.org/">0000-0002-9058-8076</datacite:nameIdentifier>

  
<datacite:affiliation>University of Veterinary Medicine Vienna</datacite:affiliation>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Pratscher, Barbara</datacite:creatorName>

  
<datacite:givenName>Barbara</datacite:givenName>

  
<datacite:familyName>Pratscher</datacite:familyName>

  
<datacite:nameIdentifier nameIdentifierScheme="ORCID" schemeURI="https://orcid.org/">0000-0001-5291-1365</datacite:nameIdentifier>

  
<datacite:affiliation>University of Veterinary Medicine Vienna</datacite:affiliation>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Gotic, Ivana</datacite:creatorName>

  
<datacite:givenName>Ivana</datacite:givenName>

  
<datacite:familyName>Gotic</datacite:familyName>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Freund, Patricia</datacite:creatorName>

  
<datacite:givenName>Patricia</datacite:givenName>

  
<datacite:familyName>Freund</datacite:familyName>

  
<datacite:affiliation>University of Veterinary Medicine Vienna</datacite:affiliation>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Hahn, Rainer</datacite:creatorName>

  
<datacite:givenName>Rainer</datacite:givenName>

  
<datacite:familyName>Hahn</datacite:familyName>

  
<datacite:nameIdentifier nameIdentifierScheme="ORCID" schemeURI="https://orcid.org/">0000-0001-5654-5032</datacite:nameIdentifier>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Himmler, Gottfried</datacite:creatorName>

  
<datacite:givenName>Gottfried</datacite:givenName>

  
<datacite:familyName>Himmler</datacite:familyName>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Brandt, Sabine</datacite:creatorName>

  
<datacite:givenName>Sabine</datacite:givenName>

  
<datacite:familyName>Brandt</datacite:familyName>

  
<datacite:nameIdentifier nameIdentifierScheme="ORCID" schemeURI="https://orcid.org/">0000-0002-3837-3422</datacite:nameIdentifier>

  
<datacite:affiliation>University of Veterinary Medicine Vienna</datacite:affiliation>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Burgener, Iwan Anton</datacite:creatorName>

  
<datacite:givenName>Iwan Anton</datacite:givenName>

  
<datacite:familyName>Burgener</datacite:familyName>

  
<datacite:nameIdentifier nameIdentifierScheme="ORCID" schemeURI="https://orcid.org/">0000-0002-8516-0040</datacite:nameIdentifier>

  
<datacite:affiliation>University of Veterinary Medicine Vienna</datacite:affiliation>

  
</datacite:creator>

  
</datacite:creators>

  
<dc:publisher>MDPI</dc:publisher>

  
<resourceType resourceTypeGeneral="literature" uri="http://purl.org/coar/resource_type/c_18cf">text</resourceType>

  
<dc:language>eng</dc:language>

  
<dc:description xml:lang="en">Given the high prevalence of intestinal disease in humans and animals, there is a strong need for clinically relevant models recapitulating gastrointestinal systems, ideally replacing in vivo models in accordance with the principles of the 3R. We established a canine organoid system and analysed the neutralising effects of recombinant versus natural antibodies on Clostridioides difficile toxins A and B in this in vitro system. Sulforhodamine B cytotoxicity assays in 2D and FITC-dextran barrier integrity assays on basal-out and apical-out organoids revealed that recombinant, but not natural antibodies, effectively neutralised C. difficile toxins. Our findings emphasise that canine intestinal organoids can be used to test different components and suggest that they can be further refined to also mirror complex interactions between the intestinal epithelium and other cells.</dc:description>

  
<datacite:subjects>
  
<datacite:subject xml:lang="en">Organoids</datacite:subject>

  
<datacite:subject xml:lang="en">Clostridioides Difficile</datacite:subject>

  
<datacite:subject xml:lang="en">sIgA</datacite:subject>

  
<datacite:subject xml:lang="en">Neutralisation</datacite:subject>

  
<datacite:subject xml:lang="en">Toxin</datacite:subject>

  
</datacite:subjects>

  
<licenseCondition uri="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</licenseCondition>

  
<file mimeType="application/pdf" objectType="fulltext">https://phaidra.vetmeduni.ac.at/api/object/o:1377/download</file>

  
<datacite:alternateIdentifiers>
  
<datacite:alternateIdentifier alternateIdentifierType="DOI">10.3390/ijms24043867</datacite:alternateIdentifier>

  
</datacite:alternateIdentifiers>

  
<datacite:relatedIdentifiers>
  
<datacite:relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://phaidra.vetmeduni.ac.at/o:605</datacite:relatedIdentifier>

  
</datacite:relatedIdentifiers>

  
<dc:format>application/pdf</dc:format>

  
<dc:source>International Journal of Molecular Sciences 24(4) (2023)</dc:source>

  
<citationTitle>International Journal of Molecular Sciences</citationTitle>

  
<citationVolume>24</citationVolume>

  
<citationIssue>4</citationIssue>

  
<datacite:sizes>
  
<datacite:size>2.76 MB</datacite:size>

  
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<datacite:dates>
  
<datacite:date dateType="Issued">2023</datacite:date>

  
</datacite:dates>

  
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