Organ-on-a-Chip: A Roadmap for Translational Research in Human and Veterinary Medicine

Title (eng)
Organ-on-a-Chip: A Roadmap for Translational Research in Human and Veterinary Medicine
Author
Surina Surina
Clinical Department for Small Animals and Horses, Division of Small Animal Internal Medicine, Univesity of Veterinary Medicine Vienna, 1210 Vienna, Austria
Author
Aleksandra Chmielewska
Clinical Department for Small Animals and Horses, Division of Small Animal Internal Medicine, Univesity of Veterinary Medicine Vienna, 1210 Vienna, Austria
Author
Barbara Pratscher
Clinical Department for Small Animals and Horses, Division of Small Animal Internal Medicine, Univesity of Veterinary Medicine Vienna, 1210 Vienna, Austria
Author
Patricia Freund
Clinical Department for Small Animals and Horses, Division of Small Animal Internal Medicine, Univesity of Veterinary Medicine Vienna, 1210 Vienna, Austria
Author
Alexandro Rodríguez-Rojas
Clinical Department for Small Animals and Horses, Division of Small Animal Internal Medicine, Univesity of Veterinary Medicine Vienna, 1210 Vienna, Austria
Author
Iwan Anton Burgener
Clinical Department for Small Animals and Horses, Division of Small Animal Internal Medicine, Univesity of Veterinary Medicine Vienna, 1210 Vienna, Austria
Abstract (eng)
In this review we offer a guide to organ-on-chip (OoC) technologies, covering the full experimental pipeline, from organoid derivation and culture, through microfluidic device fabrication and design strategies, to perfusion systems and data acquisition with AI-assisted analysis. At each stage, we highlight both the advantages and limitations, providing a balanced perspective that aids experimental planning and decision-making. By integrating insights from stem cell biology, bioengineering, and computational analytics, this review presents a compilation of the state of the art of OoC research. It emphasizes practical considerations for experimental design, reproducibility, and functional readouts while also exploring applications in human and veterinary medicine. Furthermore, key technical challenges, standardization issues, and regulatory considerations are discussed, offering readers a clear roadmap for advancing both foundational studies and translational applications of OoC systems.
Keywords (eng)
OrganoidsOrgan-on-chipMicrofluidicsTranslational MedicineVeterinary Models
Type (eng)
journal article
Language
[eng]
Persistent identifier
https://phaidra.vetmeduni.ac.at/o:5059
DOI
10.3390/ijms262110753
Is in series
Title (eng)
International Journal of Molecular Sciences
Volume
26
Issue
21
ISSN
1422-0067
Issued
2025
Number of pages
26
Publication
MDPI
Version type (eng)
version of record (published version)
Date issued
2025
Access rights (eng)
open access
License
CC BY 4.0 International
Rights statement (eng)
© 2025 by the authors