Molecular Detection and Quantification of Ovine Papillomavirus DNA in Equine Sarcoid

Francesca De Falco; Anna Cutarelli; Roberta Pellicanò; Sabine Brandt; Sante Roperto

doi:10.1155/2024/6453158

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Title (eng)

Molecular Detection and Quantification of Ovine Papillomavirus DNA in Equine Sarcoid

Author

Francesca De Falco

Anna Cutarelli

Roberta Pellicanò

Sabine Brandt

University of Veterinary Medicine Vienna

Sante Roperto

Abstract (eng)

Equine sarcoids are caused by infection with bovine papillomavirus (BPV) types 1, 2, and possibly 13. However, a number of sarcoids lack BPV DNA, and new potential etiological agents for sarcoid diseases need to be considered. High-performance digital droplet polymerase chain reaction (ddPCR) was used for the quantitative detection of ovine papillomavirus (OaPV) types 1-4 DNA from 63 sarcoid DNA samples collected in Austria. All samples were comparatively evaluated for OaPV DNA loads by qPCR. Conventional PCR and amplicon sequencing were used to validate the data. Of the 63 sarcoid DNA isolates, ddPCR was able to detect 22 samples harboring OaPV DNA (34.92%), whereas only five of the OaPV-positive samples were revealed by qPCR (22.72%). The differences in detection by ddPCR and qPCR were statistically significant (p< 0.05). The detected OaPV types were OaPV1, 3, and 4. Both methods failed to detect OaPV2 DNA, which could be due to the limited number of examined samples. Importantly, ddPCR detected multiple types of OaPV DNA in seven cases, whereas the qPCR failed to detect multiple infections. This study is the first to provide evidence of the presence of OaPV types 1, 3, and 4 DNA in a subset of equine sarcoids. The comparative detection approach underscores the superior sensitivity of ddPCR compared to that of qPCR.

Type (eng)

journal article

Language

[eng]

Persistent identifier

https://phaidra.vetmeduni.ac.at/o:4283

DOI

10.1155/2024/6453158

Is in series

Title (eng)

Transboundary and Emerging Diseases

Volume