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Diametral influence of deoxynivalenol (DON) and deepoxy-deoxynivalenol (DOM-1) on the growth of Campylobacter jejuni with consequences on the bacterial transcriptome
University of Veterinary Medicine Vienna
University of Veterinary Medicine Vienna
Bertrand Grenier DSM Animal Nutrition and Health, Research Center Tulln
Daniel Ruhnau University of Veterinary Medicine Vienna
University of Veterinary Medicine Vienna
Dian Schatzmayr DSM Animal Nutrition and Health, Research Center Tulln
BMC
Deoxynivalenol (DON) is a type B trichothecene mycotoxin that is commonly found in cereals and grains worldwide. The presence of this fungal secondary-metabolite raises public-health concerns at both the agriculture and food industry level. Recently, we have shown that DON has a negative impact on gut integrity, a feature also noticed for Campylobacter (C.) jejuni. We further demonstrated that DON increased the load of C. jejuni in the gut and inner organs. In contrast, feeding the less toxic DON metabolite deepoxy-deoxynivalenol (DOM-1) to broilers reduced the Campylobacter load in vivo. Consequently, it can be hypothesized that DON and DOM-1 have a direct effect on the growth profile of C. jejuni. The aim of the present study was to further resolve the nature of this interaction in vitro by co-incubation and RNA-sequencing.The co-incubation of C. jejuni with DON resulted in significantly higher bacterial growth rates from 30 h of incubation onwards. On the contrary, the co-incubation of C. jejuni with DOM-1 reduced the CFU counts, indicating that this DON metabolite might contribute to reduce the burden of C. jejuni
in birds, altogether confirming in vivo data. Furthermore, the transcriptomic profile of C. jejuni following incubation with either DON or DOM-1 differed. Co-incubation of C. jejuni with DON significantly increased the expression of multiple genes which are critical for Campylobacter growth, particularly members of the Flagella gene family, frr (ribosome-recycling factor), PBP2 futA-like (Fe3+ periplasmic binding family) and PotA (ATP-binding subunit). Flagella are responsible for motility, biofilm formation and host colonization, which may explain the high Campylobacter load in the gut of DON-fed broiler chickens. On the contrary, DOM-1 downregulated the Flagella gene family and upregulated ribosomal proteins.The results highlight the adaptive mechanisms involved in the transcriptional response of C. jejuni to DON and its metabolite DOM-1, based on the following effects: (a) ribosomal proteins; (b) flagellar proteins; (c) engagement of different metabolic pathways. The results provide insight into the response of an important intestinal microbial pathogen against DON and lead to a better understanding of the luminal or
environmental acclimation mechanisms in chickens.
Englisch
2024
Dieses Werk bzw. dieser Inhalt steht unter einer
CC BY-NC-ND 4.0 - Creative Commons Namensnennung - Nicht-kommerziell - Keine Bearbeitungen 4.0 International Lizenz.
CC BY-NC-ND 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
Trichothecenes Metabolism; Campylobacter Jejunidrug Effects Genetics Growth & development Metabolism; Animals; Transcriptome Drug effects; Chickens Microbiology; Gene Expression Regulation, Bacterial Drug Effects; Campylobacter Infections Microbiology Veterinary; Animal Feed Microbiology