Title
Cow blood - A superior storage option in forensics?
Language
English
Description (en)
Given the use of modified blood products (e.g. leucocyte depleted erythrocyte concentrates in SAG-mannitol, dehydrated blood powder, defibrinated blood), drawing blood from conscious animals while minimizing their stress is a good option to obtain blood for bloodstain pattern analysis. Nevertheless, the blood must be well described since individual differences in quality can occur, and storage will influence blood components qualitatively and quantitatively. Cow has been discussed as a suitable source of blood supply, but current data lack hematological and full rheological perspectives. This project includes the respective parameters in combination with passive drip pattern experiments during refrigerated storage in multiple study arms. Cow blood displayed a constant increase in viscosity (at high shear rate: 1000s-1), reflecting the expected reduction in red blood cell (RBC) flexibility. RBCs shrank but remained intact with very few irregular shapes, therefore there was no evidence of hemolysis. Influence of storage on stain size in passive drip pattern experiments with different substrates was minimal. However in cows, it is not hemolysis but an early change in suspension properties that indicates storage lesion. Viscosity (at low shear rate: 1s-1) of some blood samples increased three-fold (peaking at day 14), transitioning sharply to near-Newtonian (almost shear-independent) behavior thereafter. The higher this increase in viscosity, the greater the increase in the number of satellite spatter on glass. In order to ensure high quality simulations in the future, comprehensive rheological analyses to detect gradual changes in blood pseudoplasticity should be implemented in the forensic discipline of bloodstain pattern analysis.
Keywords (en)
Erythrocyte-Membrane; Oligomeric State; Bank Storage; Band-3; Viscosity; Stability; Protein
DOI
10.1016/j.heliyon.2023.e14296
Author of the digital object
Ursula  Windberger  (Medical University of Vienna)
Andreas  Sparer  (Medical University of Vienna)
Johann  Huber  (University of Veterinary Medicine Vienna)
Format
application/pdf
Size
8.0 MB
Licence Selected
CC BY 4.0 International
Type of publication
Article
Name of Publication (en)
Heliyon
Pages or Volume
12
Volume
9
Number
3
Publisher
Elsevier
Publication Date
2023
Content
Details
Object type
PDFDocument
Format
application/pdf
Created
11.05.2023 12:07:09
This object is in collection
Metadata
Veterinärmedizinische Universität Wien (Vetmeduni) | Veterinärplatz 1 | 1210 Wien - Österreich | T +43 1 25077 1414 | Web: vetmeduni.ac.at