
<resource xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:datacite="http://datacite.org/schema/kernel-4" xmlns="http://namespace.openaire.eu/schema/oaire/" xsi:schemaLocation="http://namespace.openaire.eu/schema/oaire/ https://www.openaire.eu/schema/repo-lit/4.0/openaire.xsd">
  
<datacite:identifier identifierType="URL">https://phaidra.vetmeduni.ac.at/o:5628</datacite:identifier>

  
<datacite:titles>
  
<datacite:title xml:lang="en">Bacterial extracellular vesicles as recyclable nutrient reservoirs</datacite:title>

  
</datacite:titles>

  
<datacite:creators>
  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Laimer-Digruber, Astrid</datacite:creatorName>

  
<datacite:givenName>Astrid</datacite:givenName>

  
<datacite:familyName>Laimer-Digruber</datacite:familyName>

  
<datacite:nameIdentifier nameIdentifierScheme="ORCID" schemeURI="https://orcid.org/">0000-0002-8809-2530</datacite:nameIdentifier>

  
<datacite:affiliation>University of Veterinary Medicine Vienna. Centre of Pathobiology</datacite:affiliation>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Edelbacher, Tanja V.</datacite:creatorName>

  
<datacite:givenName>Tanja V.</datacite:givenName>

  
<datacite:familyName>Edelbacher</datacite:familyName>

  
<datacite:nameIdentifier nameIdentifierScheme="ORCID" schemeURI="https://orcid.org/">0009-0004-6640-0346</datacite:nameIdentifier>

  
<datacite:affiliation>University of Veterinary Medicine Vienna. Centre of Pathobiology</datacite:affiliation>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Alinaghihossein, Masoumeh</datacite:creatorName>

  
<datacite:givenName>Masoumeh</datacite:givenName>

  
<datacite:familyName>Alinaghihossein</datacite:familyName>

  
<datacite:affiliation>University of Veterinary Medicine Vienna. Centre of Pathobiology</datacite:affiliation>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Yu, Mia S. C.</datacite:creatorName>

  
<datacite:givenName>Mia S. C.</datacite:givenName>

  
<datacite:familyName>Yu</datacite:familyName>

  
<datacite:nameIdentifier nameIdentifierScheme="ORCID" schemeURI="https://orcid.org/">0000-0003-4141-6443</datacite:nameIdentifier>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Chiang, Dapi Menglin</datacite:creatorName>

  
<datacite:givenName>Dapi Menglin</datacite:givenName>

  
<datacite:familyName>Chiang</datacite:familyName>

  
<datacite:nameIdentifier nameIdentifierScheme="ORCID" schemeURI="https://orcid.org/">0009-0008-6640-8670</datacite:nameIdentifier>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Kirchner, Benedikt</datacite:creatorName>

  
<datacite:givenName>Benedikt</datacite:givenName>

  
<datacite:familyName>Kirchner</datacite:familyName>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Wudy, Susanne I.</datacite:creatorName>

  
<datacite:givenName>Susanne I.</datacite:givenName>

  
<datacite:familyName>Wudy</datacite:familyName>

  
<datacite:nameIdentifier nameIdentifierScheme="ORCID" schemeURI="https://orcid.org/">0009-0001-6333-5233</datacite:nameIdentifier>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Tschulenk, Waltraud</datacite:creatorName>

  
<datacite:givenName>Waltraud</datacite:givenName>

  
<datacite:familyName>Tschulenk</datacite:familyName>

  
<datacite:affiliation>University of Veterinary Medicine Vienna. Centre of Pathobiology</datacite:affiliation>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Walter, Ingrid</datacite:creatorName>

  
<datacite:givenName>Ingrid</datacite:givenName>

  
<datacite:familyName>Walter</datacite:familyName>

  
<datacite:nameIdentifier nameIdentifierScheme="ORCID" schemeURI="https://orcid.org/">0000-0002-5038-188X</datacite:nameIdentifier>

  
<datacite:affiliation>University of Veterinary Medicine Vienna. Centre of Pathobiology</datacite:affiliation>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Kummer, Stefan</datacite:creatorName>

  
<datacite:givenName>Stefan</datacite:givenName>

  
<datacite:familyName>Kummer</datacite:familyName>

  
<datacite:nameIdentifier nameIdentifierScheme="ORCID" schemeURI="https://orcid.org/">0000-0002-0746-1403</datacite:nameIdentifier>

  
<datacite:affiliation>University of Veterinary Medicine Vienna. VetCore</datacite:affiliation>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Ludwig, Christina</datacite:creatorName>

  
<datacite:givenName>Christina</datacite:givenName>

  
<datacite:familyName>Ludwig</datacite:familyName>

  
<datacite:nameIdentifier nameIdentifierScheme="ORCID" schemeURI="https://orcid.org/">0000-0002-6131-7322</datacite:nameIdentifier>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Přibyl, Jan</datacite:creatorName>

  
<datacite:givenName>Jan</datacite:givenName>

  
<datacite:familyName>Přibyl</datacite:familyName>

  
<datacite:nameIdentifier nameIdentifierScheme="ORCID" schemeURI="https://orcid.org/">0000-0003-1232-3694</datacite:nameIdentifier>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Pfaffl, Michael W.</datacite:creatorName>

  
<datacite:givenName>Michael W.</datacite:givenName>

  
<datacite:familyName>Pfaffl</datacite:familyName>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Ehling-Schulz, Monika</datacite:creatorName>

  
<datacite:givenName>Monika</datacite:givenName>

  
<datacite:familyName>Ehling-Schulz</datacite:familyName>

  
<datacite:nameIdentifier nameIdentifierScheme="ORCID" schemeURI="https://orcid.org/">0000-0001-7384-0594</datacite:nameIdentifier>

  
<datacite:affiliation>University of Veterinary Medicine Vienna. Centre of Pathobiology</datacite:affiliation>

  
</datacite:creator>

  
</datacite:creators>

  
<dc:publisher>Springer</dc:publisher>

  
<resourceType resourceTypeGeneral="literature" uri="http://purl.org/coar/resource_type/c_6501">journal article</resourceType>

  
<datacite:rights rightsURI="http://purl.org/coar/access_right/c_abf2">open access</datacite:rights>

  
<dc:language>eng</dc:language>

  
<dc:description xml:lang="en">Bacterial extracellular vesicles (EVs) are known to mediate intercellular communication, virulence, and immune modulation. Here we show that bacteria can utilise EVs also as recyclable nutrient reservoirs. Using Bacillus cereus as a model organism, we demonstrate that EVs exhibit distinct dynamics depending on growth conditions: EVs produced in complex nutrient-rich media undergo time-dependent degradation, while those produced in defined nutrient-limited conditions remain stable and accumulate. We observe similar EV degradation patterns in Staphylococcus aureus. Time-resolved multi-omics profiling reveals that EVs containing the lipid sphingomyelin undergo progressive degradation. Using pharmacological inhibition, knockout mutants, and enzymatic complementation, we show that this process is driven by secreted sphingomyelinase (SMase). This enzyme contributes to degradation of sphingomyelin-containing EVs, thereby releasing their biomolecular cargo which can be used as a nutrient source. Growth assays confirm that SMase-mediated EV degradation provides a growth advantage when nutrients become depleted, thus establishing EVs as dynamic nutrient reservoirs.</dc:description>

  
<licenseCondition uri="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</licenseCondition>

  
<file accessRightsURI="http://purl.org/coar/access_right/c_abf2" mimeType="application/pdf" objectType="fulltext">https://phaidra.vetmeduni.ac.at/api/object/o:5628/download</file>

  
<datacite:alternateIdentifiers>
  
<datacite:alternateIdentifier alternateIdentifierType="DOI">10.1038/s41467-026-71463-3</datacite:alternateIdentifier>

  
</datacite:alternateIdentifiers>

  
<datacite:relatedIdentifiers>
  
<datacite:relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://phaidra.vetmeduni.ac.at/o:605</datacite:relatedIdentifier>

  
</datacite:relatedIdentifiers>

  
<dc:format>application/pdf</dc:format>

  
<dc:source xml:lang="en">Nature Communications</dc:source>

  
<dc:source>issn:2041-1723</dc:source>

  
<version uri="http://purl.org/coar/version/c_970fb48d4fbd8a85">VoR</version>

  
<citationTitle>Nature Communications</citationTitle>

  
<citationVolume>17</citationVolume>

  
<citationIssue>1</citationIssue>

  
<datacite:sizes>
  
<datacite:size>2.97 MB</datacite:size>

  
</datacite:sizes>

  
<datacite:dates>
  
<datacite:date dateType="Issued">2026</datacite:date>

  
</datacite:dates>

  
</resource>