https://phaidra.vetmeduni.ac.at/o:5460 Fasciola hepatica in alpine dairy farming: prevalence trends, risk factors and associations with Salmonella Dublin seropositivity Schuler, Severin Severin Schuler University of Veterinary Medicine Vienna. Centre of Pathobiology Hofer, Kerstin Kerstin Hofer Wittek, Thomas Thomas Wittek 0000-0001-6113-8458 University of Veterinary Medicine Vienna. Clinical Centre for Ruminants, Camelids and Herd Health Management Matt, Monika Monika Matt Palmieri, Nicola Nicola Palmieri University of Veterinary Medicine Vienna. Clinical Centre for Population Medicine in Fish, Pig and Poultry Nigsch, Annette Annette Nigsch Tichy, Alexander Alexander Tichy 0000-0001-8668-3858 University of Veterinary Medicine Vienna. Shared Facility Bioinformatics and Biostatistics Joachim, Anja Anja Joachim 0000-0003-3082-6885 University of Veterinary Medicine Vienna. Centre of Pathobiology Dünser, Michael Michael Dünser Mader, Christian Christian Mader Hinney, Barbara Barbara Hinney 0000-0001-7757-1002 University of Veterinary Medicine Vienna. Centre of Pathobiology Springer journal article open access eng Fasciola hepatica impairs animal health and productivity in ruminants and has been associated with Salmonella Dublin infection, a zoonotic pathogen in cattle that causes diarrhea, abortions, and pneumonia. This work reassessed F. hepatica seroprevalence in Tyrolean dairy herds and investigated associations with alpine pasturing, the breed, S. Dublin occurrence and milk performance. In late autumn/early winter 2023, bulk tank milk samples from 3645 farms were tested using a commercial ELISA test (IDEXX Fasciolosis Verification Test, Montpellier, France). Binary logistic regression and linear mixed models were used to evaluate risk factors and production effects. Overall, 86.1% of herds were positive for Fasciola antibodies - an increase of 13.1% compared to 2005. Alpine pasturing was a significant risk factor (odds ratio [OR] 4.7), and S. Dublin-positive farms had higher odds of Fasciola-infection (OR = 9.0). In non-alpine grazing herds, all models showed that Fasciola-infection was significantly associated with reduced milk yield, while no such associations were observed in the population with alpine pasture management. However, breed-specific analysis among alpine-pastured cows showed significant negative associations on milk yield in Simmental (p = 0.041) and Brown Swiss (p = 0.039). These results reveal an increasing F. hepatica prevalence in Tyrol and highlight alpine pasturing as a major risk factor. The observed production losses and the strong link with S. Dublin emphasize the parasite`s broader economic and public health importance for dairy farms in the European Alps. From a practical perspective, there is a clear need for region-specific F. hepatica control strategies in alpine dairy systems, with particular focus on pasture management and herd-level surveillance. Fasciola hepatica; Alpine grazing dairy breed Bulk tank milk production Salmonella Dublin Risk factors Epidemiology http://creativecommons.org/licenses/by/4.0/ https://phaidra.vetmeduni.ac.at/api/object/o:5460/download 10.1186/s12917-026-05383-1 https://phaidra.vetmeduni.ac.at/o:605 application/pdf BMC Veterinary Research issn:1746-6148 VoR BMC Veterinary Research 22 1 1.75 MB 2026