Severin Schuler (Centre of Pathobiology, Department of Biological Sciences and Pathobiology, Departments, University of Veterinary Medicine Vienna) Severin Schuler Kerstin Hofer Kerstin Hofer Thomas Wittek (Clinical Centre for Ruminants, Camelids and Herd Health Management, Clinical Department for Farm Animals and Food System Transformation, Departments, University of Veterinary Medicine Vienna) Thomas Wittek Monika Matt Monika Matt Nicola Palmieri (Clinical Centre for Population Medicine in Fish, Pig and Poultry, Clinical Department for Farm Animals and Food System Transformation, Departments, University of Veterinary Medicine Vienna) Nicola Palmieri Annette Nigsch Annette Nigsch Alexander Tichy (Shared Facility Bioinformatics and Biostatistics, Department of Biological Sciences and Pathobiology, Departments, University of Veterinary Medicine Vienna) Alexander Tichy Anja Joachim (Centre of Pathobiology, Department of Biological Sciences and Pathobiology, Departments, University of Veterinary Medicine Vienna) Anja Joachim Michael Dünser Michael Dünser Christian Mader Christian Mader Barbara Hinney (Centre of Pathobiology, Department of Biological Sciences and Pathobiology, Departments, University of Veterinary Medicine Vienna) Barbara Hinney Springer 2026 Fasciola hepatica impairs animal health and productivity in ruminants and has been associated with Salmonella Dublin infection, a zoonotic pathogen in cattle that causes diarrhea, abortions, and pneumonia. This work reassessed F. hepatica seroprevalence in Tyrolean dairy herds and investigated associations with alpine pasturing, the breed, S. Dublin occurrence and milk performance. In late autumn/early winter 2023, bulk tank milk samples from 3645 farms were tested using a commercial ELISA test (IDEXX Fasciolosis Verification Test, Montpellier, France). Binary logistic regression and linear mixed models were used to evaluate risk factors and production effects. Overall, 86.1% of herds were positive for Fasciola antibodies - an increase of 13.1% compared to 2005. Alpine pasturing was a significant risk factor (odds ratio [OR] 4.7), and S. Dublin-positive farms had higher odds of Fasciola-infection (OR = 9.0). In non-alpine grazing herds, all models showed that Fasciola-infection was significantly associated with reduced milk yield, while no such associations were observed in the population with alpine pasture management. However, breed-specific analysis among alpine-pastured cows showed significant negative associations on milk yield in Simmental (p = 0.041) and Brown Swiss (p = 0.039). These results reveal an increasing F. hepatica prevalence in Tyrol and highlight alpine pasturing as a major risk factor. The observed production losses and the strong link with S. Dublin emphasize the parasite`s broader economic and public health importance for dairy farms in the European Alps. From a practical perspective, there is a clear need for region-specific F. hepatica control strategies in alpine dairy systems, with particular focus on pasture management and herd-level surveillance. PDFDocument eng 2026-08-26T08:34:57.742521265Z 2026 Fasciola hepatica; Alpine grazing dairy breed Bulk tank milk production Salmonella Dublin Risk factors Epidemiology 1836186 b application/pdf http://creativecommons.org/licenses/by/4.0/