phaidra.vetmeduni.ac.at o:5451 DOI 10.3390/organoids5010002 Decellularized Extracellular Matrix for Organoids Development and 3D Bioprinting Organoids are three-dimensional multicellular structures that mimic key aspects of native tissues consisting ideal tools to study organ development and pathophysiology when incorporated in customized bioscaffolds. In vivo, the extracellular matrix (ECM) maintains tissue integrity and regulates cell adhesion, migration, differentiation, and survival through biochemical and mechanical signals. Tissue-derived decellularized extracellular matrix (dECM) can preserve organ-specific biochemical signals and cell-adhesive motifs, creating a bioactive environment that supports physiologically relevant organoid growth. 3D bioprinting technology marks a transformative phase in organoid research by enhancing the structural and functional complexity of organoid models and expanding their application in pharmacology and regenerative medicine. These systems enhance tissue modeling and drug testing while adhering to the principles of animal replacement, reduction, and refining (3Rs) in research. Remaining challenges include donor variability, limited mechanical stability, and the lack of standardized decellularization protocols that can be addressed by adopting quality and safety metrics. The combination of dECM-based biomaterials and 3D bioprinting holds great potential for the development of human-relevant, customizable, and ethically sound in vitro models for regenerative medicine and personalized therapies. In this review, we discuss the latest (2021–2025) developments in applying extracellular matrix bioprinting techniques to organoid technology, presenting examples for the most commonly referenced organoid types. eng Decellularized Extracellular Matrix Organoids Bioink 3D Bioprinting 2026-08-24T07:54:59.437Z LOMv1.0 Author BEGIN:VCARD VERSION:3.0 N:Gkantzou;Elena; FN:Elena Gkantzou X-ORCID:https://orcid.org/0000-0002-1043-8095 END:VCARD BEGIN:VCARD VERSION:3.0 N:Rodríguez-Rojas;Alexandro; FN:Alexandro Rodríguez-Rojas X-ORCID:https://orcid.org/0000-0002-4119-8127 END:VCARD BEGIN:VCARD VERSION:3.0 N:Chmielewska;Aleksandra; FN:Aleksandra Chmielewska END:VCARD BEGIN:VCARD VERSION:3.0 N:Pratscher;Barbara; FN:Barbara Pratscher X-ORCID:https://orcid.org/0000-0001-5291-1365 END:VCARD BEGIN:VCARD VERSION:3.0 N:Surina;Surina; FN:Surina Surina END:VCARD BEGIN:VCARD VERSION:3.0 N:Freund;Patricia; FN:Patricia Freund X-ORCID:https://orcid.org/0009-0007-6398-6953 END:VCARD BEGIN:VCARD VERSION:3.0 N:Burgener;Iwan A.; FN:Iwan A. Burgener X-ORCID:https://orcid.org/0000-0002-8516-0040 END:VCARD https://w3id.org/kim/hcrt/scheme https://w3id.org/kim/hcrt/text Textdokument Text LOMv1.0 yes https://creativecommons.org/licenses/by/4.0 application/pdf 611362 https://phaidra.vetmeduni.ac.at/o:5451 https://phaidra.vetmeduni.ac.at/api/object/o:5451/thumbnail LOMv1.0 discipline