University of Veterinary Medicine Vienna Decellularized Extracellular Matrix for Organoids Development and 3D Bioprinting Organoids are three-dimensional multicellular structures that mimic key aspects of native tissues consisting ideal tools to study organ development and pathophysiology when incorporated in customized bioscaffolds. In vivo, the extracellular matrix (ECM) maintains tissue integrity and regulates cell adhesion, migration, differentiation, and survival through biochemical and mechanical signals. Tissue-derived decellularized extracellular matrix (dECM) can preserve organ-specific biochemical signals and cell-adhesive motifs, creating a bioactive environment that supports physiologically relevant organoid growth. 3D bioprinting technology marks a transformative phase in organoid research by enhancing the structural and functional complexity of organoid models and expanding their application in pharmacology and regenerative medicine. These systems enhance tissue modeling and drug testing while adhering to the principles of animal replacement, reduction, and refining (3Rs) in research. Remaining challenges include donor variability, limited mechanical stability, and the lack of standardized decellularization protocols that can be addressed by adopting quality and safety metrics. The combination of dECM-based biomaterials and 3D bioprinting holds great potential for the development of human-relevant, customizable, and ethically sound in vitro models for regenerative medicine and personalized therapies. In this review, we discuss the latest (2021–2025) developments in applying extracellular matrix bioprinting techniques to organoid technology, presenting examples for the most commonly referenced organoid types. en TEXT journal article Wissenschaftlicher Artikel Articolo scientifico Text journal article Documento PDF Articolo scientifico Text Wissenschaftlicher Artikel Decellularized Extracellular Matrix Organoids Bioink 3D Bioprinting 2026 2026 Elena Gkantzou (Clinical Centre for Small Animal Health and Research, Clinical Department for Small Animals and Horses, Departments, University of Veterinary Medicine Vienna) Alexandro Rodríguez-Rojas (Clinical Centre for Small Animal Health and Research, Clinical Department for Small Animals and Horses, Departments, University of Veterinary Medicine Vienna) Aleksandra Chmielewska (Clinical Centre for Small Animal Health and Research, Clinical Department for Small Animals and Horses, Departments, University of Veterinary Medicine Vienna) Barbara Pratscher (Clinical Centre for Small Animal Health and Research, Clinical Department for Small Animals and Horses, Departments, University of Veterinary Medicine Vienna) Surina Surina (Clinical Centre for Small Animal Health and Research, Clinical Department for Small Animals and Horses, Departments, University of Veterinary Medicine Vienna) Patricia Freund (Clinical Centre for Small Animal Health and Research, Clinical Department for Small Animals and Horses, Departments, University of Veterinary Medicine Vienna) Iwan A. Burgener (Clinical Centre for Small Animal Health and Research, Clinical Department for Small Animals and Horses, Departments, University of Veterinary Medicine Vienna) MDPI