phaidra.vetmeduni.ac.at o:5115 DOI 10.1111/gfs.70019 Influence of Manure Application Techniques on the Microbial Content of Grass and Grass Silage Field observations of visible dry manure residues in organically fertilised grassland, especially in drought areas in Bavaria (Germany), raised questions about whether different manure application methods influence the microbial composition in grass and in silages. Therefore, the aim of this study was to investigate if and to which extent three manure application methods (broadcast, trailing shoe and disc injector) and a control (mineral fertiliser) influence the microbial quality of grass and grass silage. The following samples were taken in two trial years (2020–2021): Soil (n = 16), manure (n = 10), wilted-chopped grass (n = 96) and grass silage samples (n = 80). The laboratory methods used were cultivation and qPCR. The comparison between the test groups showed no significant difference in the number of aerobic bacteria, lactic acid bacteria, Lactobacillus spp., Enterobacteriaceae, E. coli and yeasts in all sample types. The Clostridia load in soil and grass before fertilisation was similar in all test groups. After fertilisation, grass samples from plots fertilised with the disc injector method had statistically significantly lower Clostridia (2.6 log10 cfu/g) than samples from the trailing shoe (3.3 log10) and the broadcast (3.2 log10) but higher than the control group (1.7 log10) (p < 0.05). Clostridia counts in silages were between 3.7 and 3.9 log10 for the manure treatments and 3.3 log10 for the control. Except for the Clostridia levels in the grass, the results of this study indicate that the grass and silage from the three manure application methods were of similar microbial quality. eng Broadcast Disc Injector Feed Hygiene Slurry Soil Trailing Shoe 2026-05-08T08:28:33.573Z LOMv1.0 Author BEGIN:VCARD VERSION:3.0 N:Dorn‐In;Samart; FN:Samart Dorn‐In X-ORCID:https://orcid.org/0000-0002-7693-7217 END:VCARD BEGIN:VCARD VERSION:3.0 N:Geißler;Hanna; FN:Hanna Geißler END:VCARD BEGIN:VCARD VERSION:3.0 N:Harms;Katrin; FN:Katrin Harms X-ORCID:https://orcid.org/0009-0006-4628-5744 END:VCARD BEGIN:VCARD VERSION:3.0 N:Guldimann;Claudia; FN:Claudia Guldimann X-ORCID:https://orcid.org/0000-0002-9895-750X END:VCARD BEGIN:VCARD VERSION:3.0 N:Spiekers;Hubert; FN:Hubert Spiekers X-ORCID:https://orcid.org/0000-0002-8666-7839 END:VCARD BEGIN:VCARD VERSION:3.0 N:Diepolder;Michael; FN:Michael Diepolder END:VCARD BEGIN:VCARD VERSION:3.0 N:Schwaiger;Karin; FN:Karin Schwaiger X-ORCID:https://orcid.org/0000-0001-5317-6892 END:VCARD https://w3id.org/kim/hcrt/scheme https://w3id.org/kim/hcrt/text Textdokument Text LOMv1.0 yes https://creativecommons.org/licenses/by/4.0 application/pdf 1074036 https://phaidra.vetmeduni.ac.at/o:5115 https://phaidra.vetmeduni.ac.at/api/object/o:5115/thumbnail LOMv1.0 discipline