https://phaidra.vetmeduni.ac.at/o:5031 IMAGE001: A new livestock multispecies SNP array to characterize genomic variation in European livestock gene bank collections Crooijmans, R. P. M. A. R. P. M. A. Crooijmans 0000-0001-8108-9972 Wageningen University & Research, Animal Breeding and Genomics, Wageningen, The Netherlands Gonzalez Prendes, R. R. Gonzalez Prendes Wageningen University and Research, Animal Breeding and Genomics, Wageningen, The Netherlands Colli, L. L. Colli Facoltà di Scienze Agrarie, Alimentari e Ambientali/DIANA Dipartimento di Scienze Animali, Della Nutrizione e Degli Alimenti, Università Cattolica del Sacro Cuore, Piacenza, Italy Del Corvo, M. M. Del Corvo Facoltà di Scienze Agrarie, Alimentari e Ambientali/DIANA Dipartimento di Scienze Animali, Della Nutrizione e Degli Alimenti, Università Cattolica del Sacro Cuore, Piacenza, Italy Barbato, M. M. Barbato Facoltà di Scienze Agrarie, Alimentari e Ambientali/DIANA Dipartimento di Scienze Animali, Della Nutrizione e Degli Alimenti, Università Cattolica del Sacro Cuore, Piacenza, Italy Somenzi, E. E. Somenzi Facoltà di Scienze Agrarie, Alimentari e Ambientali/DIANA Dipartimento di Scienze Animali, Della Nutrizione e Degli Alimenti, Università Cattolica del Sacro Cuore, Piacenza, Italy Tosser‐Klopp, G. G. Tosser‐Klopp 0000-0003-0550-4673 GenPhySE, Université de Toulouse, INRAE, ENVT, Castanet, Tolosan, France Meszaros, G. G. Meszaros 0000-0002-5937-0060 BOKU University Ajmone‐Marsan, P. P. Ajmone‐Marsan Facoltà di Scienze Agrarie, Alimentari e Ambientali/DIANA Dipartimento di Scienze Animali, Della Nutrizione e Degli Alimenti, Università Cattolica del Sacro Cuore, Piacenza, Italy Weigend, S. S. Weigend Friedrich‐Loeffler‐Institut, Institute of Farm Animal Genetics, Neustadt‐Mariensee, Germany Wallner, Barbara Barbara Wallner 0000-0003-4159-0695 Department of Biomedical Sciences and Pathobiology, Animal Breeding and Genetics, University of Veterinary Medicine Vienna, Vienna, Austria McCue, M. E. M. E. McCue University of Minnesota, College of Veterinary Medicine, St Paul, Minnesota, USA Orlando, L. L. Orlando Centre d'Anthropobiologie et de Génomique de Toulouse, CNRS UMR 5288, Université Paul Sabatier, Toulouse, France Bradley, D. D. Bradley Trinity College Dublin, University of Dublin, Dublin, Ireland Hiemstra, S. J. S. J. Hiemstra Centre for Genetic Resources, the Netherlands (CGN) of Wageningen University & Research, Wageningen, The Netherlands Schokker, D. D. Schokker Wageningen Bioveterinary Research, Lelystad, The Netherlands Peynot, N. N. Peynot Université Paris-Saclay, INRAE, AgroParisTech, GABI, Jouy- en-Josas, France Stella, A. A. Stella Institute of Agricultural Biology and Biotechnology, National Research Council (IBBA- CNR), Milan, Italy Restoux, G. G. Restoux Université Paris-Saclay, INRAE, AgroParisTech, GABI, Jouy- en-Josas, France Groenen, M. A. M. M. A. M. Groenen Wageningen University & Research, Animal Breeding and Genomics, Wageningen, The Netherlands Tixier‐Boichard, M. M. Tixier‐Boichard Université Paris-Saclay, INRAE, AgroParisTech, GABI, Jouy- en-Josas, France Wiley journal article open access eng Molecular genetic characterization of genetic resources is essential to study biodiversity. Whereas whole genome sequencing is still relatively expensive, low density SNP arrays offer a cost‐effective and standardized solution. However, most of the current arrays are species specific. Their high SNP density often exceeds diversity mapping requirements and remains too costly for many genetic resource managers. The IMAGE H2020 project aimed at developing a low‐cost multispecies SNP array to facilitate mapping of the genetic diversity in samples stored in gene banks and in vivo (on farm) traditional populations. This farm animal multispecies array contains approximately 10 K SNPs per species. The species included are cattle, sheep, goat, horse, pig, and chicken. We developed and tested this array on many samples from each of the six species. We describe here the SNP coverage and informativity across 253 breeds. We show that the array can be used to cluster local breeds according to history and genetic diversity. We illustrate its use for parentage testing. The array is publicly available at a reasonable price if ordered in multiples of 384 samples, leading to an overall cost of genotyping of approximately 15 euros per sample. Biodiversity Farm Animals Multispecies SNP Array http://creativecommons.org/licenses/by/4.0/ https://phaidra.vetmeduni.ac.at/api/object/o:5031/download 10.1111/age.70039 https://phaidra.vetmeduni.ac.at/o:605 application/pdf Animal Genetics issn:0268-9146 VoR Animal Genetics 56 5 1.52 MB 2025